Effectiveness of Ethyl Acetate Extract of Endophytic Fungi in Soursop Leaves towards the Growth of Mammary Tumor Induced by 7,12-dimethylbenz(α)anthracene in Female Rats
Journal Title: Annual Research & Review in Biology - Year 2017, Vol 18, Issue 5
Abstract
Aims: This research aims to investigate the effectiveness of ethyl acetate extract of endophytic fungi isolated Sir G5 from soursop leaf (Annona muricata L.) in vivo breast tumor growth using Sprague-dawley (SD) female strains induced by 7,12-dimethylbenz(α)anthracene (DMBA). Study Design: This study was an in vivo experiment using a completely randomized design method (RAL). Place and Duration of Study: This research was conducted from July 2016 to February 2017 in Microbiology Laboratory of Biology Research Center, Indonesian Institute of Sciences (LIPI), Cibinong-Bogor, and Research Laboratory of Biochemistry Department of Bogor Agricultural University. Methodology: Twenty-four female rats were divided into six groups; normal group (administered per os (p.o) with aquadest 2 ml for twenty two weeks), negative control (-) DMBA (administered intraperitoneal (i.p.) with 20 mg/kg body weight (bw) of 7,12-dimethylbenz(α)anthracene), positive control (+) Doxo (administered i.p with 20 mg/kg bw of 7,12-dimethylbenz(α)anthracene and p.o. with 2 µg/20 0 g bw of Doxorubicin), DI (administered i.p. with 20 mg/kg bw of 7,12-dimethylbenz(α)anthracene and p.o. with extract of 20 mg/kg bw isolate Sir G5), DII (administered i.p. with 20 mg/kg bw of 7,12- dimethylbenz(α) anthracene and p.o. with 40 mg/kg bw extract of isolate Sir G5) and DIII (administered i.p. with 20 mg/kg b.w.of 7,12- dimethylbenz(α)anthracene and with 120 mg/kg bw of extract of isolate Sir G5). Results: The result showed that body weight rats was not significantly different at each group (P>0.05). The quantity and volume of tumor samples from isolat Sir G5 extract treatment groups was significantly lower (P<0.05) than negative control DMBA and positive control doxo. Conclusion: Overall, it can be concluded that the ethyl acetate extract of endophytic fungi isolated Sir G5 from soursop leaf (Annona muricata L.) can inhibit the growth of rat breast tumor particulary on the treatment dose DI (20 mg/Kg bw).
Authors and Affiliations
C. I. Asyura, A. E. Z. Hasan, Hasim . , H. Julistiono, Husnawati . , N. Bermawie, E. I. Riyanti
Keywords
Related Articles
- EP ID EP313506
- DOI 10.9734/ARRB/2017/34656
- Views 105
- Downloads 0
Amino Acid Composition of Sour-milk Drink with Encapsulated Probiotics
At present time, there has been a growing interest in the consumption of probiotic food products. This study presents the results of the determination of amino acids in sour-milk drink with encapsulated Lactobacillus cas...
Creek`s Aquaculture Techniques in Rangamati Hill Tracts of Bangladesh
An experiment was conducted in nine suitable (average .10±2 ha) creeks of Rangamati hill tracts Bangladesh viz., of Langadu, Naniarchar and Sadar Upazila for evaluating growth, survival and production performance of Indi...
Molecular Cloning and Expression Analysis of Bovine Alpha-tocopherol Transfer Protein (α-TTP)
Aims: To understand the mechanisms by which cattle circulate and accumulate vitamin E, we cloned the cDNA for bovine α-tocopherol transfer protein (α-TTP) and examined its expression in different tissues. Methodology:...
European Sea Bass (Dicentrarchus labrax L. 1758) As a Sentinel Species in Europe to Study the Effects of Contaminants
Aim: The current study was designed to evaluate whether European Sea Bass (Dicentrarchus labrax L. 1758) can be used as a sentinel species to test contaminants. For this aim the acute lethality (96 h) of anionic surfacta...
Evaluation of Antioxidant and Free Radical Scavenging Activity of Tagetes patula
In present study, the preliminary screening of Tagetes patula contains many phytochemicals. These phytochemicals are able to reduce the oxidative stress in living organisms under adverse conditions. T. patula contains hi...