Effectiveness of Tuberculosis Smear Microscopy in Laboratory Diagnosis of Mycobacterium tuberculosis in Resource Constrained Settings
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2015, Vol 9, Issue 11
Abstract
Aim: To determine the correlation of accuracy of direct smear microscopy compared with BACTEC MGIT 960. Design: The study prospectively compare direct smear microscopy with BACTEC MGIT 960 using the reference standard, Lowenstein Jensen culture. Place and Duration: The study was conducted in Zankli Medical Centre, Abuja, between November 2004 and July 2005. Methodology: 340 suspected patients for Mycobacterium tuberculosis referred from direct observation therapy clinics located in six different government owned health facilities were referred to our facility. These patients; male (192) and female (148) were between the age of 10 and 64 years old. Three sputa samples were collected over two consecutive days and direct smear microscopy and culture were performed on these samples. Results: When compared with the reference standard, BACTEC MGIT 960 has a sensitivity and specificity of 100.0% and 56.4% respectively, and a negative predictive value of 100.0%; indicating the proportion of AFB negative participants were actually not infected with M. tuberculosis when tested with BACTEC MGIT 960. The sensitivity of direct microscopy was significantly lower than BACTEC MGIT 960 (84.9% versus 100%, p<0.001) and the specificity was significantly higher (96.6% versus 56.4%, p<0.001). Conclusions: For the purpose of effectiveness of tuberculosis program in developing countries, direct smear microscopy may still be relevant in the diagnosis of Mycobacterium tuberculosis.
Authors and Affiliations
Ameh James, Kingsley Ochei, Nnamdi Emenyonu, Lovett Lawson
Keywords
Related Articles
- EP ID EP347890
- DOI 10.9734/BJMMR/2015/6714
- Views 85
- Downloads 0
High Prevalence of Malnutrition among the Above Thirteen with Primary Pyomyositis in Northern Uganda
Aim: To determine the prevalence of malnutrition and its association with primary pyomyositis among patients and controls who were age and sex matched. Study Design and Setting: A case-control study was conducted at Gulu...
The Effect of Gum Arabic on Energy Balance Genes in Mouse Liver
Aims: Energy homeostasis is regulated by a complex neuroendocrine system including appetite regulatory hypothalamus peptides and adipocytes derived peripheral signals. The aim was to study the effects of Gum Arabic on fa...
A Qualitative Content Analysis of Breast Cancer Narratives Hosted Through the Medium of Social Media
Aims: Social media and networking host a significant amount of breast cancer related information. Breast cancer accounts for the largest number of new internet postings amongst the top four cancers. The objective of this...
An Update on Cephalometrics among Nigerians: Ascertaining Prevalent Jaw Patterns
Aims: Cephalometric norms vary between populations as well as among ethnicities within a known population. This study presents an update on cephalometric findings among Nigerians as well as the prevalent jaw patterns amo...
Severe Alopecia Complicating Systemic Sclerosis
Aims: To describe a case of systemic sclerosis (SSc) associated with severe alopecia areata (AA) responsive to topical and systemic treatments, including vasoactive and immunosuppressive drugs (mycophenolate mofetil). Pr...