Effectiveness reproduction of Cannabis sativa L. from seeds with low germination and viability in vitro conditions
Journal Title: «Таврійський науковий вісник» - Year 2018, Vol 100, Issue 2
Abstract
Hemp seed (Cannabis sativa L.) is capable of germination no more than 3–4 years when it stored under normal conditions of the warehouse or laboratory. A small number of hybrid seeds is formed as a result of hybridization. The phenomenon of inbred depression of the generative organs is in the self-pollinated hemp lines of later generations (starting with I5–I6), that is why seeds are characterized by low seed germination and viability. To obtain and preserve valuable genotypes of hemp from seeds with low germination and viability, the method of reproduction in vitro culture conditions was developed by us. It was found that the addition of 0.4 mg/l gibberellic acid and 4.0 mg/L succinic acid to the Murashige and Skoog culture medium increases seed germination by 7.5–24%.
Authors and Affiliations
С. В. Міщенко
Keywords
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