Effects of Co-Administration of Bone Marrow Stromal Cells and L-Carnitine on The Recovery of Damaged Ovaries by Performing Chemotherapy Model in Rat
Journal Title: International Journal of Fertility & Sterility - Year 2019, Vol 13, Issue 3
Abstract
Background L-carnitine (Lc) as a type of flavonoid antioxidants and bone marrow stromal cells (BMSCs) as a type of mesenchymal stem cells may recover damaged ovaries. It seems that Lc has favorable effects on differentiation, increasing lifespan and decreasing apoptosis in BMSCs. The aim of this study was to investigate effects of co-ad- ministration of BMSC+Lc on damaged ovaries after creating a chemotherapy model with cyclophosphamide in rats. Materials and Methods In this experimental study, cyclophosphamide was intraperitoneally (IP) injected to forty female wistar rats for 14 days, in terms of chemotherapy-induced ovarian destruction. The rats were then randomly divided into four groups: control, Lc, BMSCs and co-administration of BMSC+Lc. Injection of BMSCs into bilateral ovaries and intraperitoneal injection of Lc were performed individually and together. Four weeks later, levels of se- rum estradiol (E2) and follicle-stimulating hormone (FSH) using enzyme-linked immunosorbent assay (ELISA) kit, number of ovarian follicles at different stages using hematoxylin and eosin (H&E) staining and expression of ovarian Bcl-2 and Bax proteins using western blot were assessed. Results Co-administration of BMSC+Lc increased E2 and decreased FSH levels compared to the control group (P<0.001). The number of follicles was higher in the co-administrated group compared to the control group (P<0.001). Co-administration of BMSC+Lc increased Bcl-2 protein level, decreased Bax protein level and increased Bcl-2/Bax ratio (P<0.001). Conclusion The effect of co-administration of BMSC+Lc is probably more effective than the effect of their separate administration on the recovery of damaged ovaries by chemotherapy.
Authors and Affiliations
Sam Zarbakhsh, Robabeh Safari, Hamid Reza Sameni, Behpour Yousefi, Manouchehr Safari, Nasrin Khanmohammadi, Parisa Hayat
Keywords
Related Articles
- EP ID EP606187
- DOI 10.22074/ijfs.2019.5725
- Views 142
- Downloads 0
Differential Effect of Medium on the Ratio of ICM/TE of Bovine<br /> Embryos in a Co-culture System
Screening of two closed mutations in CFTR gene of Iranian infertile men with non-obstructive azoospermia
The genetic association between cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations and male infertility due to congenital bilateral absence of vas deferens (CBAVD) is well established. Mutant CFTR,...
Relationship between Quality of Life, Relationship Beliefs and Attribution Style in Infertile Couples
Background Many infertile couples experience psychological distress and suffer from impaired quality of life. Gen- erally, when couples are dealing with uncontrolled events such as infertility, it is important to manage...
Comparison of The Effects of A Positive Reappraisal Coping Intervention and Problem-Solving Skills Training on Depression during The Waiting Period of The Result of Intrauterine Insemination Treatment: A Randomized Control Trial
Background The outcomes of fertility treatments are unpredictable, and levels of depressive symptoms increase in patients during the waiting period of the result of intrauterine insemination (IUI) treatment. The aim of t...
Evaluation of Tumor Necrosis Factor Alpha Polymorphism Frequencies in Endometriosis
Background The pro-inflammatory cytokine, tumor necrosis factor-alpha (TNF-α), is a pathogenic element for a number of disorders. Previous studies have reported that the -1031 T/C and -238 G/A polymorphisms in the promot...