Effects of Cotinine on Human Gingival Fibroblast Migration
Journal Title: Dentistry – Open Journal - Year 2015, Vol 2, Issue 1
Abstract
Nicotine has a deleterious impact on gingival fibroblast cell viability, adhesion, and migration. Less is known regarding the effect of cotinine, the main metabolite of nicotine, on such processes. The objective of this study was to determine if cotinine affects the adhesion or migration of Human Gingival Fibroblasts (HGF) in culture. HGF were treated with nicotine or cotinine at several concentrations and dose-response cytotoxicity was determined by MTT assay. The effects of nicotine and cotinine on HGF adhesion were measured colorimetrically and cell migration was determined using the scratch wound assay. The number of HGF oriented parallel to the wound edge at 24 hours was counted using phase contrast images. Data were analyzed using ANOVA and Dunnet’s multiple comparison post-test. At the highest concentrations of cotinine (640 ng/ml) and nicotine (400 µg/ml) both HGF survival and cell adhesion were significantly inhibited (p<0.01). By scratch wound assay HGF migration from the wound edge at 24 hours was significantly inhibited by 320 ng/ml (p<0.001) and 640 ng/ml (p<0.001) cotinine, and by 400 µg/ml (p<0.01) nicotine. HGF migration was significantly inhibited by 80 ng/ml (p<0.05), 320 ng/ml (p<0.01) and 640 ng/ml (p<0.001) cotinine and by 100 µg/ml (p<0.01), 200 µg/ml (p<0.01) and 400 µg/ml (p<0.001) nicotine at 48 hours. Significantly more HGF were oriented parallel to wound edge with pre treatment of 10 ng/ml cotinine or 50 µg/ml nicotine before wounding (p<0.001). In HGF exposed to nicotine (400 µg/ml) or cotinine (640 ng/ml), cell survival, cell adhesion, and migration were significantly decreased, but cell polarity was not affected. These concentrations are within ranges of serum levels in smokers, providing evidence that multiple cellular aspects of wound healing are compromised in tobacco users.
Authors and Affiliations
Michelle A. Wheater
Keywords
Related Articles
- EP ID EP551934
- DOI 10.17140/DOJ-2-106
- Views 156
- Downloads 0
Discover A New Smile-Transform Your Life
Your smile plays a major role in how you perceive yourself, as well as in the impressions you make on the people around you. Surveys have shown that more than any other physical feature-eyes, hair or body-the smile is wh...
Inverted Periosteal Technique – A Solution to Multiple Teeth Recession
Background: The periosteum being a highly cellular connective tissue also has a good regenerative potential. This quality makes it a suitable for use as autogenous graft. Here, is technique, the inverted periosteal techn...
Frequency of Post-Instrumentation Symptoms Using Hand Files Versus Rotary Protapers
Introduction Controversy among dentists exists to use conventional hand files or contemporary rotary protapers for intracanal instrumentation. The controversy arose because of variable findings on post-instrumentation pa...
Combined Orthodontic with Implant to Rehabilitate Vertical Dimension of Occlusion
The control of Occlusal Vertical Dimension (OVD)1 is a demanding task for dentists. Establishment of proper occlusal vertical dimension in prosthetic treatment is a important task for clinical procedure.2 No methods are...
The Antimicrobial Activity of Pomegranate Polyphenol Extract (POMx) Lozenges in a Saliva-Derived Biofilm Model System
The ellagitannin type polyphenols present in Pomegranate extracts (POMx) have been associated with numerous health benefits including antibacterial activities. Despite their antibacterial potency, however, these purified...