Effects of different nutritional factors on culturable species diversity of soil bacteria
Journal Title: 广东农业科学 - Year 2018, Vol 45, Issue 8
Abstract
"By adding six different nutrient factors to the modified VL55 medium, a large number of dilutions were used to isolate bacteria from three soil samples collected from three regions of Hebei, Qinghai, and Yunnan. The phylogenetic analysis of the 16S rRNA gene sequences was performed on the strains isolated using six different nutrient factors to compare the species diversity of culturable bacteria. The results showed that the bacterial species diversity obtained from the addition of nutritional factors was significantly higher than that of the control; 5 genera were isolated from the soil sample 1 control, 22 genera were isolated from the experimental group with the addition of six nutritional factors; soil sample 2 control was isolated two genera, 17 genera were isolated from the experimental group; 6 genera were isolated from the soil sample 3 control, and 15 genera were isolated from the experimental group. The combination of culture media with different nutrient factors was added to isolate their unique bacterial populations. Among them, the strain HBUM200206 isolated from the soil sample of Qinghai Province had the closest genetic relationship with Dongia mobilis LM22T, and the 16S rRNA gene sequence similarity was 94.58%, which may be a potential new genera of Rhodospirillaceae. In addition, the 16S rRNA gene sequence similarity of the 12 strains and the closest relatives of the type strains were all less than 98.65%, which may be potential new species."
Authors and Affiliations
Feng-xia JING, Xiu-min ZHANG
Keywords
Related Articles
- EP ID EP601502
- DOI 10.16768/j.issn.1004-874X.2018.08.010
- Views 71
- Downloads 0
Exploration on the transformation and development of agricultural SMEs under the background of supply side structural reform—A case of a leading agricultural enterprise in Heilongjiang
"China's Agricultural SMEs are facing seriously overcapacity and relatively high cost which caused low value-added space，industrial competitiveness and other issues. Aiming at the existing problems，this paper systemati...
Bioinformatics analysis on MTP1 genes in the AC genomes of Brassicas
"Genome databases of Brassica rapa, Brassica oleracea and Brassica napus were used to identify the MTP1 genes in the whole genomes, the AtMTP1 gene sequences in Arabidopsis thaliana were used as reference. Blastn alignme...
Effects of fertilization and clipping on the biochemical characteristics of Carex brevicuspis
Carex brevicuspis is one of the most widely distributed plants in Dongting Lake. It has large biomass and high nutritional value, and is an excellent animal husbandry feed resource. In order to reveal the physiological r...
Study on the removal ability of five species of Zingiberaceae to sewage
"In order to study the sewage purification ability of five species of Zingiberaceae，including Hedychium coronarium，Alpinia. spp，Hedychium. sp，H. qingchengense，and H. flavescens. The dynamic changes of nitrogen（ N）...
Forecast and suggestion of vegetable production in Guangxi based on Gray Prediction Model GM（1, 1）
Based on the gray system theory and the data of vegetable production in Guangxi from 2008 to 2014, this paper established a gray forecasting model GM (1,1) of vegetable production in Guangxi province and carried out resi...