Effects of gamma rays on rat testis tissue according to the morphological parameters and immunohistochemistry: radioprotective role of silymarin
Journal Title: Electronic Physician - Year 2017, Vol 9, Issue 6
Abstract
OBJECTIVE: To determine the radioprotective effects of Silymarin in adult male Sprague-Dawley rats irradiated with γ-rays. METHODS: The present experimental study was performed in Tehran University of Medical Sciences, Tehran, Iran from December 2009 to March 2010. The study was performed on 40 rats, which were randomly and equally divided into four groups: 1) control group: neither received Silymarin nor irradiated with γ-rays; 2) γ-irradiation group: testis region exposed to 2Gy of γ-rays; 3) Silymarin & γ-irradiation: rats received 100 mg/kg of Silymarin 24hrs before exposure to 2Gy of γ-rays; 4) Silymarin & γ-irradiation: rats received 200 mg/kg of Silymarin 24hrs before exposure to 2Gy of γ-rays. After animal experiments and preparing the tissue sections, different histological and histomorphological parameters of seminiferous tubules and the biological characteristics of Leydig cells were evaluated applying quantitative assessment, Johnson scoring, and Leydig cell apoptosis assay by TUNEL method. The data were analyzed applying ANOVA and Tukey's post hoc test, using SPSS software (V.19). RESULTS: Irradiation of 2 Gy γ-rays to the testis of the rats significantly affected the frequency of spermatogonia, primary spermatocyte, round spermatid, spermatozoa, seminiferous tube and lumen diameters, thickness of the epithelium, Leydig cell nuclear diameter and volume, epithelium height, and apoptotic cells (p<0.05). However, administration of Silymarin improved the mentioned parameters specifically in 200 mg/kg of dosage. CONCLUSION: Silymarin could act as a potent radioprotector and it can be used in modulation as well as improvement to radiation therapy to prevent male reproductive function, specifically seminiferous tubules in an animal model; however, its molecular mechanism is still not clear and needs more molecular researches
Authors and Affiliations
Mohsen Marzban, Maryam Anjamshoa, Parnia Jafari, Hossien Masoumi, Reza Ahadi, Daryoush Fatehi
Keywords
Related Articles
- EP ID EP321681
- DOI 10.19082/4524
- Views 67
- Downloads 0
Management of Mallet Fracture by Closed Extension-Block Pinning – A case based review of a novel technique
Close reduction by extension-block K-wire fixation for acute mallet fracture is a relatively novel mode of treatment which is based on two sound orthopedic principles – stable arc splinting and early protected motion. Di...
Use of Artificial Neural Networks (ANNs) for the Analysis and Modeling of Factors That Affect Occupational Injuries in Large Construction Industries.
Occupational injuries as a workforce's health problem are very important in large-scale workplaces. Analysis and modeling the health-threatening factors are good ways to promote the workforce's health and a fundamental s...
Effective factors on menstrual health among female students in Bam city: a qualitative study
BACKGROUND: Menarche is an independent puberty event in a girl's life, and is associated with physical growth, ability in marriage and fertility. Therefore, poor menstrual health can be a major determinant of morbidity,...
Evaluation of mouse embryos produced in vitro after electromagnetic waves exposure; Morphometric study.
Today, the use of electromagnetic waves in medical diagnostic devices, such as magnetic resonance imaging (MRI), has increased, and many of its biological effects have been reported. The aim of the present study was to a...
Assessment of the diagnostic accuracy of double inversion recovery sequence compared with FLAIR and T2W_TSE in detection of cerebral multiple sclerosis lesions
BACKGROUND: Multiple sclerosis (MS) is a demyelinating disease of the central nervous system. MRI has an important role in early diagnosis of MS within diagnostic criteria. AIM: To determine the diagnostic value of the...