Effects of Hydrogen Sulphide on the Isolated Perfused Rat Heart
Journal Title: Sultan Qaboos University Medical Journal - Year 2011, Vol 11, Issue 2
Abstract
Objectives: Hydrogen sulphide has been identifed as a gas signalling molecule in the body, and has previously been shown to have vasorelaxant properties. Te aim of the study was to investigate the effects of sodium hydrosulphide (NaHS), a hydrogen sulphide donor, on heart rate (HR), left ventricular developed pressure (LVDP) and coronary flow (CF) in the isolated perfused rat heart. Methods: A Langendorff isolated heart preparation was used to investigate the effect of a dose range of sodium hydrosulphide, in the presence and absence of inhibitors, on heart rate, left ventricular developed pressure and coronary flow. Results: Sodium hydrosulphide caused a signifcant decrease in heart rate at a concentration of 10-3 M (P <0.001). Tis decrease was partially inhibited by glibenclamide, a K ATP channel blocker (P <0.05); L-NAME, a nitric oxide synthase inhibitor (P <0.001), and methylene blue (P <0.001), but not by H-89, a protein kinase A inhibitor. Sodium hydrosulphide signifcantly increased coronary flow at concentrations of 10-4 – 10-3M (P <0.05). Tis response was signifcantly increased in the presence of L-NAME (P <0.001) and methylene blue (P <0.001), whereas H-89 inhibited the increase in coronary flow due to sodium hydrosulphide (P <0.001). Sodium hydrosulphide signifcantly decreased LVDP at all concentrations (P <0.001). In the presence of glibenclamide and H-89, the time period of the decrease in LVDP due to sodium hydrosulphide was extended (P <0.001), whereas methylene blue and L-NAME caused a signifcant reduction in the response to sodium hydrosulphide (P <0.05, P <0.01 respectively). Conclusion: Sodium hydrosulphide reduced heart rate and LVDP, and increased coronary flow in the isolated perfused rat heart; however, the mechanisms of action could not be fully elucidated.
Authors and Affiliations
Afthab Hussain| Biomolecular Sciences, Faculty of Health & Life Sciences, Coventry University, Coventry, U, Helen Maddock| Biomolecular Sciences, Faculty of Health & Life Sciences, Coventry University, Coventry, U, Hajar Al-Rajaibi| Department of Physiology, Sultan Qaboos University Hospital, Muscat, Oman, Ray J Carson| Dept. of Medical & Social Care Education, Leicester Medical School, University of Leicester, Leicester, UK
Keywords
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