Effects of Hydrogen Sulphide on the Isolated Perfused Rat Heart
Journal Title: Sultan Qaboos University Medical Journal - Year 2011, Vol 11, Issue 2
Abstract
Objectives: Hydrogen sulphide has been identifed as a gas signalling molecule in the body, and has previously been shown to have vasorelaxant properties. Te aim of the study was to investigate the effects of sodium hydrosulphide (NaHS), a hydrogen sulphide donor, on heart rate (HR), left ventricular developed pressure (LVDP) and coronary flow (CF) in the isolated perfused rat heart. Methods: A Langendorff isolated heart preparation was used to investigate the effect of a dose range of sodium hydrosulphide, in the presence and absence of inhibitors, on heart rate, left ventricular developed pressure and coronary flow. Results: Sodium hydrosulphide caused a signifcant decrease in heart rate at a concentration of 10-3 M (P <0.001). Tis decrease was partially inhibited by glibenclamide, a K ATP channel blocker (P <0.05); L-NAME, a nitric oxide synthase inhibitor (P <0.001), and methylene blue (P <0.001), but not by H-89, a protein kinase A inhibitor. Sodium hydrosulphide signifcantly increased coronary flow at concentrations of 10-4 – 10-3M (P <0.05). Tis response was signifcantly increased in the presence of L-NAME (P <0.001) and methylene blue (P <0.001), whereas H-89 inhibited the increase in coronary flow due to sodium hydrosulphide (P <0.001). Sodium hydrosulphide signifcantly decreased LVDP at all concentrations (P <0.001). In the presence of glibenclamide and H-89, the time period of the decrease in LVDP due to sodium hydrosulphide was extended (P <0.001), whereas methylene blue and L-NAME caused a signifcant reduction in the response to sodium hydrosulphide (P <0.05, P <0.01 respectively). Conclusion: Sodium hydrosulphide reduced heart rate and LVDP, and increased coronary flow in the isolated perfused rat heart; however, the mechanisms of action could not be fully elucidated.
Authors and Affiliations
Afthab Hussain| Biomolecular Sciences, Faculty of Health & Life Sciences, Coventry University, Coventry, U, Helen Maddock| Biomolecular Sciences, Faculty of Health & Life Sciences, Coventry University, Coventry, U, Hajar Al-Rajaibi| Department of Physiology, Sultan Qaboos University Hospital, Muscat, Oman, Ray J Carson| Dept. of Medical & Social Care Education, Leicester Medical School, University of Leicester, Leicester, UK
Keywords
Related Articles
- EP ID EP14049
- DOI -
- Views 281
- Downloads 10
Complications of Central Venous Catheterisation Breakage of guidewire-a disaster averted
Central venous catheterisation (CVC) is a common bedside invasive procedure done in medical practice. Even though it is a safe procedure when done with ultrasound guidance, difculties and complications do occur even in...
Relapse of Multiple Myeloma Presenting as Lower Lip Numbness
Multiple myeloma (MM) is an uncommon malignancy characterised by the proliferation of clonal plasma cells. Tere are few published reports describing the extramedullary presentation of MM manifesting primarily in the he...
Comparative Study of Ambulatory Blood Pressure Monitoring and Clinic Blood Pressure Measurement in the Risk Assessment and Management of Hypertension
Blood pressure (BP) measurements taken in a physician’s clinic do not represent readings throughout the day. Ambulatory blood pressure monitoring (ABPM) overcomes this problem by providing multiple readings with mini...
Estimates of Average Glandular Dose with Auto-modes of X-ray Exposures in Digital Breast Tomosynthesis
Objectives: Te aim of this research was to examine the average glandular dose (AGD) of radiation among different breast compositions of glandular and adipose tissue with auto-modes of exposure factor selection in digi...
Unusual Presentation of Oral Squamous Cell Carcinoma in a Young Woman
Oral squamous cell carcinoma (OSCC) is the most common oral malignant neoplasm, mainly affecting individuals over 50 years old with a history of tobacco and alcohol use. Te occurrence of this oral cancer in individual...