Effects of lipoic acid on spleen oxidative stress after LPS administration.
Journal Title: Pharmacological Reports - Year 2013, Vol 65, Issue 1
Abstract
Background: Bacterial endotoxin (lipopolysaccharide - LPS) is a strong modulator of the immune system that plays a crucial role in the pathogenesis of endotoxic shock. The aim of the study was to investigate the effects of lipoic acid (LA) on oxidative stress markers in spleen homogenates obtained from LPS-induced endotoxic shock rats. Methods: The animals were treated with saline or lipoic acid (LA) (60 or 100 mg/kg b.w. iv) 30 min before or 30 min after LPS administration (30 mg/kg b.w. iv). Five hours after LPS, LA or saline administration, the animals were euthanized and their spleens were isolated for measurements. Results: The LPS-treated animals developed oxidative stress, indicated by a significant increase in thiobarbituric acid-reactive substances (TBARS) and hydrogen peroxide (H2O2) concentrations (p < 0.001) as well as an insignificant decrease in the level of sulfhydryl groups (-SH groups) and the glutathione redox ratio [reduced glutathione (GSH) to oxidized glutathione (GSSG) ratio] (p < 0.02) as compared with control group. Treatment with LA (60 or 100 mg/kg) before or after LPS administration resulted in an increase in -SH group content (p < 0.01) and a decrease in TBARS and H2O2 concentration in the spleen as compared with LPS group (p < 0.001). LA (60 or 100 mg/kg) before LPS administration decreased the level of GSSG (p < 0.05) and increased the level of GSH in spleen homogenates (p < 0.05), resulting in an increase in the GSH/GSSG ratio compared with the LPS group (p < 0.01). It also improved the LPS-induced increase in the spleen weight (SW) to body weight (BW) ratio (p < 0.001 vs. control). Conclusion: The present results have shown that LA is endowed with antioxidant properties that are protective in the spleen against the deleterious actions of Gram-negative bacterial endotoxin.
Authors and Affiliations
Anna Gorąca, Halina Huk-Kolega, Paulina Kleniewska, Aleksandra Piechota-Polańczyk, Beata Skibska
Keywords
Related Articles
- EP ID EP114841
- DOI -
- Views 79
- Downloads 0
Effects of co-administration of fluoxetine and risperidone on properties of peritoneal and pleural macrophages in rats subjected to the forced swimming test.
Literature data show that administration of atypical antipsychotic drug, risperidone (RIS), enhances antidepressive action of fluoxetine (FLU). As antidepressive treatments also regulate immune functions, we examined whe...
Effects of n-3 polyunsaturated fatty acids on depressive symptoms, anxiety and emotional state in patients with acute myocardial infarction.
Background: Our aim was to assess whether an early introduced n-3 polyunsaturated fatty acids (n-3 PUFA) supplementation affects depression symptoms, anxiety and emotional state in patients with acute myocardial infarcti...
Inhibitors of type 2 sodium glucose co-transporters - a new strategy for diabetes treatment.
In the last few years, the type 2 sodium glucose co-transporters (SGLT2) have been the subject of particular attention as a new, potent group of anti-diabetic drugs. SGLT2 inhibitors block glucose reabsorption in the kid...
Fluorescence studies of homooligomerization of adenosine A(2A) and serotonin 5-HT(1A) receptors reveal the specificity of receptor interactions in the plasma membrane.
The concept that G protein-coupled receptors (GPCRs) function as oligomers has been widely accepted, however, different methodologies often used to study the phenomenon of GPCR interactions do not allow, as yet, for any...
COX-2 as a target for cancer chemotherapy.
Cyclooxygenase-1 and -2 (COX-1/2) catalyze the initial step in the formation of prostaglandins. Very recently their role in carcinogenesis has become more evident. They influence apoptosis, angiogenesis, and invasion, an...