Effects of NCoRsiRNA on proliferation and osteogenic differentiation of mesenchymal stem cells in high glucose microenvironmen
Journal Title: INTERNATIONAL JOURNAL OF CURRENT RESEARCH - Year 2016, Vol 8, Issue 12
Abstract
Background: Chronic hyperglycemia enhances osteoporosis in diabetic patients mainly by inhibiting osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Nuclear receptor co repressor (NCoR) has been reported to negatively modulate osteogenic differentiation of Rat BMSCs in a standard culture medium, conversely, its knockdown promoted osteogenic differentiation. In this study, we investigated the effects of NCoR knockdown on proliferation and osteogenic differentiation of MSCs under a high glucose microenvironment. Methods: Cells from Wistar rats were isolated, transfected with NCoR small interfering RNA (NCoRsiRNA), cultured in various glucose concentrations (5.5, 16.5,25 and 35mmol/L) and then cell proliferation determined using methyl thiazolyltetrazolium (MTT). Osteogenic differentiation of cells cultured in normal (5.5mmol/L) and high glucose (25mmol/L) was determined through quantitative changes in mineralization (calcium accumulation), alkaline phosphatase (ALP) activity and expression of osteoblast marker genes including Runx2, osteocalcin (OCN), Osterix, bone sailoprotein (BSP) and osteopontin (OPN)using quantitative Real Time PCR. Results: NCoR knockdown in MSCs cultured in high glucose (25mmol/L) inhibited cell proliferation but resulted in increased Calcium accumulation, alkaline phosphatase (ALP) activity and elevated mRNA expression of all osteoblast related genes. Conclusion: NCoR knockdown inhibited proliferation but promoted osteogenic differentiation of BMSCs under a high glucose micro-environment.
Authors and Affiliations
Ignatius Chomba, Hongwei Gao, Deguo Xing and Sambiani K. Mingzhi Gong
Keywords
Related Articles
- EP ID EP404991
- DOI -
- Views 82
- Downloads 0
Case report of neuroradiological imaging - maple syrup urine disease
Maple Syrup Urine Disease (MSUD) is a rare inherited autosomal recessive disorder caused by a deficiency of the branched-chain alpha-keto acid dehydrogenase complex (BCKDC), leading to a buildup of the branched-chain ami...
Guidelines for supportive oral care in cancer therapy patients
There are various treatment options for head and neck cancers consisting of surgery, chemotherapy and radiotherapy. Each of these are treatment options associated with a number of considerations related to treatment of...
The Factors Which Effect Mortality and Morbidity Rate In Patients During Postoperative Period
Background: Parallel to the advanced surgical techniques, the critically ill postoperative surgical patients are increasing in intensive care units (ICU). Mortality and morbidity of these patients in ICU are multifactori...
Gastroprotective effects of loranthus micranthus and acalypha wilkesiana leaves extracts on experimentally induced ulcer models in rats
The anti-ulcer activities of Loranthus miranthus leaf extract (LOME) and Acalypha wilkesiana leaf extract (AWLE) were investigated in rats in which ulcer was induced with indomethacin (40mg/kg). Results obtained indicate...
Practical learning and theory-practice gap as perceived by nursing students
Background: Practical learning is one of the important concerns that helps the awareness of nursing students’ practice in a clinical setting and it is important to link theoretical knowledge to practical skills. Nursing...