Effects of new antiepileptic drugs and progabide on the mitogen-induced proliferative activity of mouse splenocytes.
Journal Title: Pharmacological Reports - Year 2008, Vol 60, Issue 6
Abstract
Classical antiepileptic drugs are known to affect immune system activity, although the effects of new generation anticonvulsants on T- and B-cell-mediated immunity remain unknown. Therefore, in the present study, we compared a selection of new antiepileptic drugs with classical ones in terms of their effects on the proliferative activity of lymphocytes stimulated by concanavalin A(Con A) and lipopolysaccharide (LPS). Felbamate (3 x 10(-6) - 10(-4) M) was the most potent in inhibiting [(3)H]-thymidine incorporation in C57BL/6 mouse spleen cells stimulated by Con A and LPS. Treatment of the cells with stiripentol (3 x 10(-5) and 10(-4) M) and loreclezole (10(-4) M) suppressed the proliferative activity of splenocytes both after Con A and LPS stimulation. Tiagabine (3 x 10(-5) M and 10(-4) M) inhibited the Con A-induced cell proliferation, whereas the effect of LPS was attenuated only by the highest concentration of this drug (10(-4) M). Progabide showed immunosuppressive effects at concentrations of 3 x 10(-5) and 10(-4) M or only 10(-4) M after LPS or Con A stimulation, respectively. No effect on the immune parameters was observed after lamotrigine treatment. On the other hand, the Con A-induced proliferation of splenocytes was enhanced by carbamazepine (10(-5) - 10(-4) M) and sodium valproate (5 x 10(-4) - 3 x 10(-3) M). Neither carbamazepine nor sodium valproate affected the LPS-induced proliferation. These data indicate that some new antiepileptic drugs, especially felbamate at pharmacological concentrations, may suppress the mitogen-stimulated proliferative activity of mouse splenocytes. In contrast, two classical anticonvulsants (carbamazepine and sodium valproate) stimulated T-cell-mediated immunity. The above differences in the effects of particular antiepileptic drugs on the immune response may play roles in both their therapeutic efficiency and undesired effects.
Authors and Affiliations
Agnieszka Basta-Kaim, Bogusława Budziszewska, Monika Leśkiewicz, Marta Kubera, Grzegorz Jagła, Wojciech Nowak, Stanisław Czuczwar, Władysław Lasoń
Keywords
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