Effects of Punica Granatum Peel on Oral Bacterial Species- An In-Vitro Study.
Journal Title: International Journal of Dental Science and Innovative Research (IJDSIR) - Year 2019, Vol 2, Issue 2
Abstract
Introduction: Oral diseases such as dental caries, gingival and periodontal diseases are major oral health problems worldwide, affecting the majority of the population. Microbial plaque accumulation is proven to be the main etiologic factor in the development of both gingivitis and periodontitis. Aims and objectives: To assess the antibacterial, the anti-inflammatory, and the antioxidant activity of Punica granatum fruit peel extract by an in-vitro method. Methodology: The antibacterial activity of punica granatum fruit peel extract was evaluated by disc diffusion method and determination of minimum inhibitory concentration (MIC) against Streptococcus sanguis, Streptococcus mutans and Staphylococcus aureus by micro broth dilution technique. The anti-inflammatory activity of Punica granatum fruit peel extract was determined by lipoxygenase inhibition assay and hyaluronidase inhibition assay.The antioxidant activity of punica granatum fruit peel extract was determined by 2, 2’-azino-bis ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging assay and 2, 2 diphenyl – 1 picrylhydrazyl (DPPH) antioxidant assay. The statistical tests used are Kruskal Wallis testandStudent unpaired t-test. Results: The results showed that that the chlorhexidine exhibited a zone of inhibition with the diameter of 17.0±1.0, 14.3±3.2 and 12.0±1.7 whereas, the punica granatum peel extract exhibited a zone of inhibition with the diameter of 14.7±4.0, and 12.0±1.0 for Streptococcus mutans,Streptococcus sanguis, and Staphylococcus aureus respectively. The anti-inflammatory tests revealed that the indomethacin group showed lower percentage of lipoxygenase inhibition activity with the mean of 24.17+-19.34, as compared to punica granatum group mean of 29.44+-23, and with the mean difference of -5.27. The antioxidant assay revealed that the quercetin group had higher radical scavenging property with the mean of 37.08±34.26, as compared to Punica granatum group with the mean of 25.81±28.46 with the mean difference of 11.27. However, the results obtained were not statistically significant. Conclusion: The results obtained from the current study concluded that the punica granatum peel extract has an antibacterial, anti-inflammatory and antioxidant property under laboratory condition.
Authors and Affiliations
Dr. Latha G.
Keywords
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