Effects of salt stress on leaf protein patterns of rapeseed (Brassica napus L.) genotypes
Journal Title: Journal of Biodiversity and Environmental Sciences (JBES) - Year 2017, Vol 10, Issue 2
Abstract
Environmental stresses caused a protein disorders in plants and plants overcome to stress by altering the expression of genes which necessary for the synthesis of metabolites, structural proteins and enzymes of some metabolic pathways. To study the effect of NaCl salinity on rapeseed’s protein patterns, twelve spring genotypes were evaluated by hydroponic culture at seedling stage. Experiment was performed on three levels of salinity (0, 175, 350 mM) in three replications. In order to study protein patterns by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method, four weeks after salt stress performing and at the end of the seedling stage, leaf samples were collected. For each genotype in each stress level, proteins were extracted in three samples and electrophoresis was performed for them. 30 reproducible bands were identified by SDS-PAGE, in which 18 were polymorphic. Polymorphic bands divided to two groups. The first group had genetic origins and showed the differences among genotypes and the second one called stress-dependent and were affected by salinity. The 20.1 kDa protein bands were induced by salinity in all genotypes while the protein bands with an approximate 56.7 kDa weight were not shown under salt stress. Cluster analysis of data which obtained from protein patterns based on polymorphic bands were used to classification, which had shown high similarity with genotype grouping basis of physiological, biochemical and agronomical data.
Authors and Affiliations
Nima Dolatabadi, Mahmoud Toorchi
Keywords
Related Articles
- EP ID EP39042
- DOI -
- Views 193
- Downloads 0
Sowing date evaluation on phenological traits of canola genotypes under warm and semi arid climate (ahvaz region)
Evaluating the phenological traits of rapeseed crop provides the ability to manage planting based on optimal compatibility to the environmental condition. Thus, experiment on the basis of split plot arrangement in rand...
Prokaryotic Community Profiles of Soils from Mayon Volcano, Philippines Based on 16S Ribosomal RNA Gene Sequences
Mayon Volcano is the Philippines’ most active volcano. Despite extensive pedological, ecological and ethnobotanical studies, no published information is known about its soil microflora. In this study, to determine the mi...
Phenology, life form and leaf spectra of the vegetation of kokarai valley, district swat
Kokarai valley District Swat is rich in plant biodiversity. The plant species varies in their phenological behavior, life form and leaf size spectra. There is no previous record in the area and therefore the study was de...
Simulation of Nickel in soils affected by wastewater and sludge by using Hydrus 1D
Transport of heavy metals in contaminated soils can cause groundwater pollution. In the present study Hudrus one dimensional software was used for nickel metal transfer from soil to groundwater. Concentrations of Ni was...
Butterfly diversity varies across habitat types in Tangkoko Nature reserve North Sulawesi, Indonesia
Butterflies (Lepidoptera) are important pollinators. This study aims to analyze the diversity of butterflies in various habitat types in Tangkoko Nature Reserve (TNR) North Sulawesi, Indonesia. Butterflies were sampled i...