Effects of silencing Ki-67 gene on doxorubicin resistance of breast cancer MCF-7/DOX cells
Journal Title: Journal of Surgery : Concepts & Practice - Year 2023, Vol 28, Issue 3
Abstract
[Objective] To study the effect of Ki-67 gene silencing on doxorubicin (DOX) resistance in breast cancer MCF-7 cells. [Methods] Liposome transient transfection method and breast cancer MCF-7 cells cultured in vitro were used combined with the transfection of small interfering RNA (siRNA) and negative control (NC) siRNA. The expression of Ki-67 mRNA was detected by RT-PCR after 48 h of transfection. Ki-67 siRNA with the highest interference efficiency was used in subsequent experiments including three groups: control (CON) group without transfection, NC group transfected with MCF-7 negative control siRNA and siRNA group with MCF-7 transfected with Ki-67 siRNA. The expression of Ki-67 mRNA and related proteins in three groups were detected by RT-PCR and Western blot. MCF-7/DOX cells were treated with DOX at different concentrations. Proliferation of MCF-7/DOX cells was detected by MTT assay. [Results] After the specific siRNA was successfully transfected into MCF-7 /DOX cells, it was observed under fluorescence microscope that siRNA was evenly distributed in the cytoplasm, and transfection efficiency was more than 85%. [Results] of transfection of MCF-7 siRNA cells showed that Ki-67 mRNA expression in MCF-7 cells reduced Ki-67 at varying degrees. The expression of Ki-67 protein reduced significantly with the inhibition of 78.51% and the statistically significant difference compared with that in NC group and CON group (P < 0.05). MTT showed that the proliferation ability of MCF-7/DOX cells in vitro was inhibited significantly after Ki-67 mRNA expression interfered by siRNA. The half maximal inhibitory concentration (IC50) of DOX to MCF-7/DOX cells in siRNA group was (7.45±0.18) μmol/L, lower significantly than (55.19±2.86) μmol/L in NC group and (56.43±4.22) μmol/L in CON group. The reversal of drug resistance was 7.69 times compared with that in CON group (P < 0.05). The inhibitory effect of DOX at different concentrations on the cells in siRNA group was significantly higher than that in NC group and CON group with statistically significant difference (P < 0.05). [Conclusions] It was indicated that siKi-67 could inhibit both the expression of Ki-67 gene effectively and the proliferation of MCF-7/DOX cells, and partially reverse the DOX resistance of MCF-7/DOX cells.
Authors and Affiliations
Jun DONG, Fengming CUI, Jun LIU
Keywords
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- EP ID EP750881
- DOI 10.16139/j.1007-9610.2023.03.013
- Views 22
- Downloads 0
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