Effects of Some Environmental Variables on Urease in Germinating Chickpea (Cicer arietinum L.) Seed
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2013, Vol 9, Issue 3
Abstract
Enzyme activity is influenced by a large number of factors. Environmental conditions such as pH, temperature, salt concentration, substrate concentration, activators, and inhibitors may change the three dimensional shape of an enzyme, altering its rate of activity and/or its ability to bind substrate. The effects of such environmental factors were evaluated. The optimum pH and temperature of the purified urease were 7.2 and 48°C, respectively, using urea as substrate. The optimum substrate (urea) concentration for urease was 25 mM. The enzyme showed the highest activity when incubated for 30 min at 48[sup]0[/sup]C. EDTA, a metal chelator, decreased the enzyme activity significantly. This may be due to the removal of metal ions located on or near the active site. Divalent cations like Ba[sup]2+[/sup] and Mg[sup]2+[/sup] slightly stimulated the enzyme at a concentration of 1-3 mM whereas Na[sup]+[/sup] and K[sup]+[/sup] produced little or no effect on the activity. Ca[sup]2+[/sup] enhanced urease activity by 120.47%, while Pb[sup]2+[/sup], Cu[sup]2+[/sup], Zn[sup]2+[/sup] and Hg[sup]2+[/sup] almost completely inhibited the urease activity.
Authors and Affiliations
M. Shaela Pervin, A. Y. K. Masud Rana, N. Sana, R. Shaha
Keywords
Related Articles
- EP ID EP93475
- DOI -
- Views 122
- Downloads 0
The effect of plant defense elicitors on common bean (Phaseolus vulgaris L.) growth and yield in absence or presence of spider mite (Tetranychus urticae Koch) infestation
[b]Background [/b]The common bean plants "[i]Phaseolus vulgaris[/i] L." is frequently attacked by the two spotted spider mite (TSSM) Tetranychus urticae, causing a substantial decrease in bean plant growth and yield as...
Reduced antioxidant status for prolonged period due to repeated stress exposure in rat
The objective of the study was to find out whether or not exposure to a stressor after an initial stressful experience augments stress response. Antioxidant status was determined by measuring changes in the activities...
Dynamics of Vitamins and Phenols of Alchemilla subcrenata by Diurnal Variation of Temperature in October
Round-the-clock dynamics of phenols and vitamins В[sub]6[/sub], С, РР in the leaves of [i]Alchemilla subcrenata[/i] with a glance of thermal change was shown. The maximum of content of phenols and vitamin С was observed...
Evaluation of Oxidative Stress in Sheep Affected with Peste des petits ruminants
The aim of the investigation was to evaluate oxidative stress in sheep affected with peste des petits ruminants (PPR). Oxidative stress in the affected sheep was evaluated by determining various serum biomarkers viz. vi...
CONSERVATION LEUKOCYTES IN THE CONDITIONS OF<br /> CRYOANABIOSIS (-40°С)
Trying to meet the requirements in leukocytes the clinicists are facing the pressing problem of conservation of cells in a functionally valid condition which is possible in deep cryoanabiosis (-196°C). This conservation...