Effects of tea extracts of different fermentations on proliferation of colorectal cancer cell line HCT116
Journal Title: 广东农业科学 - Year 2018, Vol 45, Issue 9
Abstract
There is no conclusion if tea polyphenols have effect on colorectal cancer, and no report about the effect of tea polyphenols on colorectal cancer cells in different degree of fermentation (unfermented/semi-fermented/ full-fermented). Three kinds of tea polyphenol extracts of Huangshan Maofeng, Robe Tea and Keemun Black Tea were selected in this study. The effects of tea polyphenol extracts on cell growth were detected by MTS method. Cell morphological changes of colorectal cancer cell HCT116 were observed by inverted microscope after treated with three tea polyphenol extracts separately. The MTS data reflected that the proliferation of HCT116 cells could be inhibited in different degrees by these tea polyphenol extracts from three kinds of tea (P<0.05). And the IC 50 of tea polyphenol extracts to HCT116 were different according to the different fermentation degree of the tea. The morphology of the cells which were separately treated by the tea polyphenol extracts with the doses of 15, 35 μg/mL were observed. The results showed that HCT116 were undergoing different degree of apoptosis morphological changes compared with the control after treated by three kinds of tea polyphenol extracts for 72 h. The tea polyphenol extracts of Huangshan Maofeng, Robe Tea and Keemun Black Tea have inhibitory effects on HCT116 and induce the apoptosis of HCT116. The inhibition effects to HCT116 by the tea polyphenol extracts from tea are significantly different according to the different fermentation degree.
Authors and Affiliations
Yan-hui LI, Hai-yan SUN
Keywords
Related Articles
- EP ID EP602824
- DOI 10.16768/j.issn.1004-874X.2018.09.017
- Views 96
- Downloads 0
Ultrasonic-microwave-assisted extraction of total flavonoids from Seed oil residue of Passiflora edulis Sims
"The seed oil residue of Passiflora edulis Sims was used as the study object，and the flavonoids in the seed oil residue were extracted by ultrasonic-microwave-assisted methodology. First single-factor experiments were t...
Relationship between the PRV release of porcine pseudorabies and the level of gE，gB antibody
"Different samples including serum，milk， nasal swabs and so on，in different stages were collected from PRV-postitive and negative farm in present study，the level of gE and gB antibody were detected by ELISA， The res...
Expression analysis of the MAPK genes in exogenous NO induced disease resistance to Penicillium digitatum in the postharvest citrus fruits
"Nitric oxide（ NO） is an important signal molecule involved in plant defense responses. It also induces the activation of the mitogen-activated protein kinase（ MAPK）. To study the expression of MAPK genes in exogenou...
Effects of fertilization and clipping on the biochemical characteristics of Carex brevicuspis
Carex brevicuspis is one of the most widely distributed plants in Dongting Lake. It has large biomass and high nutritional value, and is an excellent animal husbandry feed resource. In order to reveal the physiological r...
Effects of pH and dosages of montmorillonite on the carbon, nitrogen, phosphorus and heavy metals adsorption from biogas slurry
"In order to save the cultivation cost and select the local cultural substrate for industrial production of potted blueberries in Guangdong, mixed matrix of three different blended formulas was designed for this study: s...