Effects of tea extracts of different fermentations on proliferation of colorectal cancer cell line HCT116
Journal Title: 广东农业科学 - Year 2018, Vol 45, Issue 9
Abstract
There is no conclusion if tea polyphenols have effect on colorectal cancer, and no report about the effect of tea polyphenols on colorectal cancer cells in different degree of fermentation (unfermented/semi-fermented/ full-fermented). Three kinds of tea polyphenol extracts of Huangshan Maofeng, Robe Tea and Keemun Black Tea were selected in this study. The effects of tea polyphenol extracts on cell growth were detected by MTS method. Cell morphological changes of colorectal cancer cell HCT116 were observed by inverted microscope after treated with three tea polyphenol extracts separately. The MTS data reflected that the proliferation of HCT116 cells could be inhibited in different degrees by these tea polyphenol extracts from three kinds of tea (P<0.05). And the IC 50 of tea polyphenol extracts to HCT116 were different according to the different fermentation degree of the tea. The morphology of the cells which were separately treated by the tea polyphenol extracts with the doses of 15, 35 μg/mL were observed. The results showed that HCT116 were undergoing different degree of apoptosis morphological changes compared with the control after treated by three kinds of tea polyphenol extracts for 72 h. The tea polyphenol extracts of Huangshan Maofeng, Robe Tea and Keemun Black Tea have inhibitory effects on HCT116 and induce the apoptosis of HCT116. The inhibition effects to HCT116 by the tea polyphenol extracts from tea are significantly different according to the different fermentation degree.
Authors and Affiliations
Yan-hui LI, Hai-yan SUN
Keywords
Related Articles
- EP ID EP602824
- DOI 10.16768/j.issn.1004-874X.2018.09.017
- Views 92
- Downloads 0
Isolation and phylogenetic analysis of goose parvovirus isolated in Guangdong province
"Two strains of goose parvovirus（ GPV） were isolated from the main goose breeding region in Guangdong province and were named as GDQY2017 and GDYJ2017，respectively. Phylogenetic analysis of VP gene showed that 2 isola...
Effects of applying sheep manure on soil properties of kyoho vineyard
"In order to improve the soil fertility of vineyard by rational application of sheep manure, and reduce the pollution of nitrogen, iron, copper and other substances in sheep manure to the environment, with fully decompos...
Study on the growth rhythm of Tapiseia siriensis at seedling stage
"Taking the Tapiseia siriensis introduced from the Wufeng county Yichang city as materials，we surveyed the seedling height，ground diameter and leaf growth in different months at the Longyuan nursery of Yanling county，...
Efficiency analysis of Zhanjiang sugar industry based on DEA Model of bank loan service
"This paper takes the Zhanjiang sugar industry in Guangdong as an example and uses the DEA method to evaluate the efficiency of the bank loan service in the sugar industry under the different industrial models and links....
Study on composting effect of excrement and green plant wastes in leisure agricultural park
Excrement and green plant waste were chosen as material in composting experiments to study the influence of bacteria agent and different proportion of excrement and green plant wastes on composting effect. The results sh...