Effects of Yishenbupi (tonifying-kidney and invigorating-spleen) prescription on the expression of renal fibrosis-associated proteins in unilateral ureteral occlusion rats
Journal Title: TMR Integrative Medicine - Year 2020, Vol 4, Issue 13
Abstract
Objective: To evaluate the effects and mechanism of the Yishenbupi (tonifying-kidney and invigorating-spleen) prescription on the expression of renal fibrosis-associated vimentin, α-SMA, and fibronectin in unilateral ureteral occlusion rats. Methods: A total of 48 SD (Sprague-Dawley) rats were randomly divided into the model, sham-operated (sham), irbesartan, and Yishenbupi groups, with 12 rats in each group. After the unilateral ureteral occlusion model was established, rats in the model and sham groups were administered normal saline, whereas rats in the Yishenbupi group were administered Yishenbupi prescription (18 g/kg/d) intragastrically and those in the irbesartan group were administered irbesartan (10 mg/kg/d) intragastrically. All rats were sacrificed 21 days later. Pathological changes in rat renal tissue were evaluated by H&E staining. The expression of vimentin, α-SMA, and fibronectin in renal tissues was detected by western blotting. Results: Compared with the sham group the model group had renal tubular epithelial cell atrophy, inflammatory cell infiltration accompanied with the proliferation of interstitial collagen fibers, fewer glomeruli, or glomerulosclerosis. Compared with the model group, significantly less renal tubular and glomerular damages, inflammatory cell infiltration, and collagen fibers were observed in different intervention groups, especially in the Yishenbupi group. Compared with the sham group, significantly higher expressions of fibrosis markers, including vimentin, α-SMA, and fibronectin, were observed in the model group. Compared with the model group, the expression of anti-fibrosis markers, including vimentin, α-SMA and fibronectin, was significantly decreased in both the irbesartan and Yishenbupi groups (P < 0.01); however, the Yishenbupi group showed higher efficacy than the irbesartan group (P < 0.05). Conclusion: The Yishenbupi prescription may improve renal fibrosis by reducing the expression of fibrosis-associated vimentin, α-SMA, and fibronectin.
Authors and Affiliations
Jue-Ying Chen,Ji-Wei Qiu,Jun-Lin Mo,Chun-Li Long,Wei Shi,Yong-Xiang Xie
Keywords
Related Articles
- EP ID EP689647
- DOI 10.12032/TMRIM202004013
- Views 194
- Downloads 0
The effects of inhaled aromatherapy on complications of hemodialysis patients: a systematic review
Objective: To evaluate the effect of inhaled aromatherapy on complications in patients undergoing hemodialysis. Methods: We search the PubMed, Web of Science, Cochrane Library, Embase and China Biomedical Literature Data...
Investigation of the effectiveness of LI4, LI10, LR3 and ST36 acupoints electroacupuncture in radialis and ulnaris nervus injuries
Background: Radial and ulnar nervus injuries are among the most common peripheral nerve injuries in veterinary medicine. In this study, it was aimed to evaluate electroacupuncture applications in radialis and ulnaris ner...
Evaluate the prevalence and trend of hepatitis B and hepatitis C in Nahavand: west of Iran, 2013–2017
Viral hepatitis is a global threat to public health and one of the leading causes of death worldwide. Often, acute viral cases in children and adults are associated with viral hepatitis A, B, C, D and E, or co-infection...
A systemic review on vitiligo (baraṣ) & role of Unani medicines towards its treatment
Vitiligo is not only a cosmetic problem, but also a social and psychological problem with the prevalence rate between 1–2 % and being highest in India. As far as etiopathogenesis of the disease is concerned, out of vari...
Effects of mindfulness-based stress reduction therapy on anxiety and depression in patients with maintenance hemodialysis: a meta analysis
Objective: This study aimed to evaluate the effects of mindfulness-based stress reduction therapy on anxiety and depression in patients with maintenance hemodialysis. Methods: CNKI, VIP, WanFang, CBM, Embase, the Cochran...