EFFICACY OF MEROPENEM AND AMIKACIN COMBINATION AGAINST METALLOBETA- LACTAMASE-PRODUCING ACINETOBACTER STRAINS

Journal Title: International Journal of Medical Laboratory Research - Year 2019, Vol 4, Issue 2

Abstract

Background: Multiple-drug resistance of Acinetobacter species cause difficulties in the treatment of infections. Due to decrease in success rates with monotherapy combinations that show synergistic effect in the treatment of MDR Acinetobacter infections is used. Objectives: The study aimed to investigate the efficacy of meropenem and amikacin combinations against metallo-beta-lactamase (MBL)-producing Acinetobacter strains isolated from clinical specimens. Method: The presence of MBL in strains was detected by gradient diffusion method (GDM). Fifty MBL-positive and 50 MBL-negative strains were included in the study. The activity of meropenem-amikacin combination against MBL-positive isolates was investigated by both GDM and the checkerboard method while the activity against MBL-negative isolates was investigated by the checkerboard method. Results: Additive or indifferent interactions between meropenem and amikacin were detected in 38 (76%) of the 50 MBL-positive strains, synergistic interactions were detected in 7(14%), and antagonistic interactions were detected in 5(10%) using GDM. Using the checkerboard method, additive or indifferent interactions between the drugs were detected in 37 (74%) and synergistic interactions in 13 (26%) of 50 MBLpositive strains while synergistic interactions were observed in 36 (72%) and additive or indifferent interactions in 14 (28%) of 50 MBL-negative strains. No antagonistic interaction was detected in the MBL-positive and MBL-negative strains using the checkerboard method. In MBL-positive strains no difference was found between the results of checkerboard and GDM. Conclusion: Based on our detection of 72% synergistic interactions between meropenem and amikacin on MBL-positive strains in the Gold Standard checkerboard assay, it is concluded that in vitro evidence supports meropenem and amikacin combination therapy against non–MBLproducing Acinetobacter spp. but further clinical studies are needed.

Authors and Affiliations

Keywords

Related Articles

IS RESISTANCE TO ETHIONAMIDE AN EXTRAPOLATION OF OTHER FIRST AND SECOND LINE ANTI- TUBERCULOSIS DRUGS?

Drug Susceptibility profile of ethionamide and other first and second line anti tuberculosis drugs revealed no significant association, indicating the resistance towards ethionamide is the result of individual effect and...

Incidence of urinary tract infection in pregnant women: observations from a tertiary care hospital

BACKGROUND: Urinary tract infection (UTI) is a common problem among the pregnant women, which can complicate the outcome of the pregnancy. The objective of this study was to identify the most common pathogens causing UTI...

STANDARD OPERATING PROCEDURE (SOP) OF – HIGH PERFORMANCE LIQUID CHROMATOGRAPHY SYSTEM (HPLC)

HPLC is a form of liquid chromatography used for separating compounds that are dissolved in solution. It is a technique for identification, quantification and purification of mixtures for analytical purposes. Application...

LEVELS OF TROPNIN AND CREATINE KINASE MB IN MYOCARDIAL INFARCTION PATIENTS

Background.Myocardial infarction (MI) commonly known as a heart attack occurs when blood flow stops to part of the heart causing damage to the heart muscle. Troponin, or troponin complex, is acomplex of three regulatory...

COLLISION TUMOR OF OVARY: SEROUS ADENOCARCINOMA AND MATURE CYSTIC TERATOMA

Collision tumor are tumors which are composed of two histologically distinct tumors in the same organ without any intermixing of two components. Simultaneous coexistence of high grade serous adenocarcinoma with mature cy...

Download PDF file
  • EP ID EP662497
  • DOI -
  • Views 77
  • Downloads 0

How To Cite

(2019). EFFICACY OF MEROPENEM AND AMIKACIN COMBINATION AGAINST METALLOBETA- LACTAMASE-PRODUCING ACINETOBACTER STRAINS. International Journal of Medical Laboratory Research, 4(2), 7-15. https://europub.co.uk/articles/-A-662497