Efficient callogenesis and Shoot organogenesis from nodal explants of Rosa hybrida L. (Lovely girl)
Journal Title: Journal of Research in Biology - Year 2012, Vol 2, Issue 4
Abstract
The callogenic and shoot organogenic potential of ornamental plant, Rosa hybrida were investigated. Callus induction and shoot regeneration were induced from nodal explants of garden plants incubated on Murashige and Skoog (MS)-medium supplemented with different concentrations of Plant Growth Regulators (PGRs). The best callus induction (80-85%) was observed on explants incubated on MS-medium supplemented with 2.0 mg l-1 6-benzyladenine (BA) and 1.5 mg l-1 BA along with 0.5 mg l-1 α-Naphthaleneacetic acid (NAA) or 0.5 mg l-1 2,4 dichlorophenoxyacetic acid (2,4 D) after four weeks of culture. When MS-medium supplemented with 1.5 mg l-1 BA alone induced 90% shoot organogenesis after 30-days following culture. 2.0 mg l-1 BA in combination with 1.0 mg l-1 Kin (Kinetin) also induced 90 % shooting. Moreover, when shoots were transferred to an elongation medium, the longest shoots (3.9 cm) were observed with similar composition of PGRs. The regenerated shoots were inoculated on rooting medium supplemented with different concentrations of Indole Butyric Acid (IBA), NAA and Indole Acetic acid (IAA) but no rooting was observed.
Authors and Affiliations
Nighat Seema, Kiran Yasmin Khan, Mohammad Zakaria, Hina Fazal
Keywords
Related Articles
- EP ID EP565589
- DOI -
- Views 105
- Downloads 0
Phytochemical and pharmacological properties of certain medicinally important species of Cucurbitaceae family – a review
Cucurbits are edible crops, belonging to the family Cucurbitaceae. They are climbers with reserves in roots and are distributed mainly in tropical zones. They are mostly moist vines and are distributed in both the easter...
Bio monitoring of air pollution around urban and industrial sites
Plants are the only living organisms which have to suffer a lot from automobiles exhaust pollution and industrial pollution because they remain static at their habitat. Experiments on air and bio monitoring were conducte...
Genetic Variation among Dwarf Gourami (Trichogaster lalia, Osphronemidae) populations using Random Amplified Polymorphic DNA(RAPD) markers
Random amplified polymorphic DNA (RAPD) was applied to generate species-specific diagnostic fragment patterns for the molecular identification of the ornamental aquarium fish species Trichogaster lalia, more commonly kno...
Comparative evaluation of hyaluronic acid production by Streptococcus thermophilus isolated from yoghurt
Hyaluronic acid (HA) is also known by the name hyaluronan. The necessity for using this fabulous material lead to investigate non-pathogenic strains which produce this material. The most non-pathogenic strain is S. therm...
The birds of Patan district of Gujarat, India
Study on the birds of Patan district was conducted during 2006-2008. Observations on bird species were recorded at two locations in each Taluka. Observations on breeding status, residential status, and utilization of sit...