Efficient callogenesis and Shoot organogenesis from nodal explants of Rosa hybrida L. (Lovely girl)
Journal Title: Journal of Research in Biology - Year 2012, Vol 2, Issue 4
Abstract
The callogenic and shoot organogenic potential of ornamental plant, Rosa hybrida were investigated. Callus induction and shoot regeneration were induced from nodal explants of garden plants incubated on Murashige and Skoog (MS)-medium supplemented with different concentrations of Plant Growth Regulators (PGRs). The best callus induction (80-85%) was observed on explants incubated on MS-medium supplemented with 2.0 mg l-1 6-benzyladenine (BA) and 1.5 mg l-1 BA along with 0.5 mg l-1 α-Naphthaleneacetic acid (NAA) or 0.5 mg l-1 2,4 dichlorophenoxyacetic acid (2,4 D) after four weeks of culture. When MS-medium supplemented with 1.5 mg l-1 BA alone induced 90% shoot organogenesis after 30-days following culture. 2.0 mg l-1 BA in combination with 1.0 mg l-1 Kin (Kinetin) also induced 90 % shooting. Moreover, when shoots were transferred to an elongation medium, the longest shoots (3.9 cm) were observed with similar composition of PGRs. The regenerated shoots were inoculated on rooting medium supplemented with different concentrations of Indole Butyric Acid (IBA), NAA and Indole Acetic acid (IAA) but no rooting was observed.
Authors and Affiliations
Nighat Seema, Kiran Yasmin Khan, Mohammad Zakaria, Hina Fazal
Keywords
Related Articles
- EP ID EP565589
- DOI -
- Views 113
- Downloads 0
Anatomical investigations in Silybum marianum (L.) Gaertn.
Anatomical studies have been carried out for various parts of species Silybum marianum (L.) Gaertn. According to the available literature very little work has been done on this aspect. Keeping this in view presently, the...
Habitat diversity, Morphological and systematic analysis of multipotential species of Aloe barbadensis Mill. (Liliaceae) from the Southern Western Ghats of Tamil Nadu, India
The present paper highlights the habitat variability, morphological features, systematic analysis and multi potentiality of Aloe barbadensis Mill. were collected from Nilgiri district of Southern Western Ghats of Tamil N...
Seasonal variation in the benthic fauna of Krishnagiri reservoir, Tamilnadu
The diversity of benthic invertebrates from in and around Krishnagiri reservoir was studied during the period of Jan 2006-June 2008. The benthic index calculated using Oligochaeta communities with environmental character...
On exposure to LC-50 of cadmium chloride, Ophiocephalaus punctatus alters the physicochemical parameters of water
Cadmium is highly toxic to aquatic biota and severely alters the biochemical, physiological and histopathological aspects of organisms. It also induces the change in physico-chemical parameters of water. It is observed t...
Studies on the suitability of certain culture media for Beauveria bassiana (Bals.) Vuill., and Verticillium lecanii (Zimm.) Viegas
The entomopathogenic fungi, Beauveria bassiana (Bals.) Vuill. and Verticillium lecanii (Zimm.) Viegas are capable of infecting a wide range of insect pests and show promise in commercialization. This laboratory study was...