ELISA and indirect immunofluorescent assays evaluation for IgM antibodies detection against Rickettsiosis
Journal Title: Revista Peruana de Medicina Experimental y Salud Publica - Year 2008, Vol 25, Issue 3
Abstract
We evaluated two modified tests of ELISA and indirect immunofluorescence (IFI) for detecting IgM based on the total lysate of Rickettsia Akari for Rickettsiosis diagnosing. 55 negative sera were used, 100 positive sera confirmed by IFI IgGTotal and 15 sera with a confirmed diagnosis for other bacterial diseases from the serum of the Instituto Nacional de Salud (Lima, Perú). IgM ELISA test had a sensitivity of 78.0%, 80.0% specificity, positive predictive value of 87.6% and negative predictive value of 66.7%, and have a 20% (3/15) cross-reaction with other bacterial aetiologies. The IFI IgM test had a sensitivity of 82.0%, specificity of 91.7%, positive predictive value of 94.3% and negative predictive value of 75.3%, with a 13% (2/15) cross-reaction. The IgM ELISA test is not useful, unlike the IFI IgM test that could be applied in diagnosing Rickettsiosis for surveillance and epidemiological studies in Peru. .
Authors and Affiliations
Elizabeth Anaya, Cecilia Morón, Patricia , Arias, Johann Chauca, Raúl Román
Keywords
Related Articles
- EP ID EP113058
- DOI -
- Views 114
- Downloads 0
Dogs susceptibility to rabies after a vaccination campaign in endemic areas of Peru.
[i][b]Objective: [/b][/i]To determine the level of immune response after a mass vaccination campaign against rabies in two areas which presented cases of canine rabies in the last years. [i][b]Materials and methods:[/b][...
Efecto Genotóxico del Dicromato de Potasio EnEritrocitos de sangre periférica de Oreochromisniloticus (tilapia)
Existen múltiples reportes del efecto genotóxico y cancerígeno del cromo VI, los seres humanos tenemos una permanente exposición a este elemento. [b][i]Objetivos. [/i][/b]Evidencias la genotoxicidad del dicromato de pota...
Microfilaria, Mansonella ozzardi
Exposición ocupacional a plomo y pesticidas organofosforados: efecto sobre la salud reproductiva masculina.
Actualmente, la exposición ocupacional a contaminantes como metales pesados y plaguicidas ha crecido por la actividad industrial, minera y agrícola. Los efectos adversos en la salud humana se presentan en el sistema resp...
Novel influenza A (H1N1): control and its impact on human rights