ELISA and indirect immunofluorescent assays evaluation for IgM antibodies detection against Rickettsiosis
Journal Title: Revista Peruana de Medicina Experimental y Salud Publica - Year 2008, Vol 25, Issue 3
Abstract
We evaluated two modified tests of ELISA and indirect immunofluorescence (IFI) for detecting IgM based on the total lysate of Rickettsia Akari for Rickettsiosis diagnosing. 55 negative sera were used, 100 positive sera confirmed by IFI IgGTotal and 15 sera with a confirmed diagnosis for other bacterial diseases from the serum of the Instituto Nacional de Salud (Lima, Perú). IgM ELISA test had a sensitivity of 78.0%, 80.0% specificity, positive predictive value of 87.6% and negative predictive value of 66.7%, and have a 20% (3/15) cross-reaction with other bacterial aetiologies. The IFI IgM test had a sensitivity of 82.0%, specificity of 91.7%, positive predictive value of 94.3% and negative predictive value of 75.3%, with a 13% (2/15) cross-reaction. The IgM ELISA test is not useful, unlike the IFI IgM test that could be applied in diagnosing Rickettsiosis for surveillance and epidemiological studies in Peru. .
Authors and Affiliations
Elizabeth Anaya, Cecilia Morón, Patricia , Arias, Johann Chauca, Raúl Román
Keywords
Related Articles
- EP ID EP113058
- DOI -
- Views 104
- Downloads 0
Evaluación de pruebas de ELISA e inmunofluorescencia indirecta para la detección de anticuerpos IgM contra Rickettsiosis
Se evaluó dos pruebas modificadas de ELISA e inmunofluorescencia indirecta (IFI) para la detección de IgM basadas en el lisado total de Rickettsia akari para el diagnóstico de Rickettsiosis. Se usaron 55 sueros negativos...
Factores asociados a lesiones cervicales o presencia del virus del papiloma humano en dos poblaciones de estudiantes de Lima.
[b]Objetivos: [/b]Determinar la prevalencia y factores asociados a lesiones cervicales o presencia del virus del papiloma humano (VPH) en mujeres estudiantes en educación superior de 18 a 26 años de Lima. [b]Materiales y...
Perceptions of health personnel and the community in relation to cultural appropriateness of maternal perinatal services in rural andean and Amazonian areas from Huanuco Region
Maternal mortality is a priority issue worldwide, most cases occur in underdeveloped countries with illiteracy, poorly accessible rural areas and with poor access and insufficient trained health personnel. [b]Objectives....
Ocular manifestations in HIV/AIDS patients in pre-HAART era.
Prospective study was performed in 821 HIV-AIDS patients evaluated in ophthalmology and infectious diseases services of a public hospital in Lima, Peru as a part of the initial evaluation to access HAART protocol treatme...
Situación sociofamiliar, valoración funcional y enfermedades prevalentes del adulto mayor que acude a establecimientos del primer nivel de atención, Callao 2006.
[b][i]Objetivos. [/i][/b]Determinar la situación sociofamiliar, valoración funcional y enfermedades prevalentes de los adultos mayores que acuden a los establecimientos de salud del primer nivel de atención de la Direcci...