Elucidation of The Microbial Community in Activated Sludge using PCR-DGGE Analysis in Arid and Semi Arid Regions of Rajasthan
Journal Title: International Journal of Scientific Research and Reviews - Year 2012, Vol 1, Issue 3
Abstract
Activated sludge is the most commonly used process to treat sewage and industrial waste water by micro organisms. The activated sludge system depends on the activities of microbial communities present in the sludge. However, exact knowledge of the microbial community structure in waste water treatment plants is limited. In this study, the bacterial diversity of activated sludge was investigated in the two waste water treatment plants by using denaturing gradient gel electrophoresis (DGGE) of PCRamplified 16S ribosomal DNA fragments. Dominant bands from DGGE profiles were excised and subjected to sequencing to identify the dominant genotypes. Sequence analysis gave insights into the identities of the predominant bacterial populations present. The DNA sequencing results indicated the microbial diversity, revealing that the dominant bacteria present in Bramhapuri waste water treatment plant is Acinetobacter sp. whereas the dominant bacteria in Pratapnagar (Delawas) waste water treatment plant is Alpha-proteobacteria. Futhermore, cluster analysis of the DGGE profiles indicated significant diversity in the bacterial community by depicting two distinct clusters for each waste water treatment plant. These data endorse the ability of PCR-DGGE method to identify and characterize bacterial community from activated sludge.
Authors and Affiliations
Shivani Chandra, Mathur N. , Nallapeta S. , Bhatnagar P.
Keywords
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