EMPHASIS ON ANTIBIOTIC OPTIMISATION IN DIFFICULT TO TREAT METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS INFECTION
Journal Title: Journal of Evolution of Medical and Dental Sciences - Year 2017, Vol 6, Issue 71
Abstract
BACKGROUND Emergence of serious infections caused by Gram-positive pathogens is increasing despite remarkable advances in antimicrobial agents. Of great concern is increasing incidence of Community acquired Methicillin Resistant Staphylococcus aureus (MRSA) infection, due to its growing frequency and its poor associated outcome. This study aims to analyse resistance pattern of Staphylococcus aureus (S. aureus) isolates in an attempt to understand prevalence of MRSA from tertiary care centre. The objective of this study is to measure the severity, antimicrobial susceptibility of S. aureus isolates from a tertiary care centre and to discuss preventive measure to control its transmission. MATERIALS AND METHODS Present study was carried out in the Department of Microbiology (Study duration- 1 yr., Jan 2015 - Dec 2015), total of 252 nonduplicate S. aureus isolates from various clinical specimens (pus, blood, sterile site fluids) were included in the study. Isolates were identified by standard microbiological techniques. All S. aureus isolates were subsequently tested for methicillin resistance by cefoxitin (30 μg) discs test on Mueller-Hinton agar (MHA). Zone diameter of ≤ 21 mm was reported as MRSA and ≥ 22 mm was considered as sensitive as per Clinical and Laboratory Standards Institute (CLSI). All isolates were further screened for susceptibility pattern by Kirby-Bauer disc diffusion method on MHA. The antibiotics used were ampicillin (AMP)10 µg; linezolid (Lz) 30 μg; vancomycin (V) 30 μg; ciprofloxacin (Cf) 5 μg; gentamicin (G) 30 μg; Azithromycin (Az) 15 µg; and Erythromycin (E) 15 µg (HiMedia, India). S. aureus ATCC 25923 was used as control strain and results were interpreted according to CLSI. RESULTS Among MRSA isolates 70/118 (59.32%), 48/118 (40.67%), 46/118 (38.98) and 58/118 (49.15%) were resistant to β-lactam, aminoglycosides, fluoroquinolones and macrolides respectively. However, lower resistance was seen in oxazolidinones 5 (4.23%) and all isolates were susceptible to glycopeptide (Vancomycin). CONCLUSION MRSA continues to be a growing burden for the health-care system due to the poor prognosis and high costs associated with this infection. Higher resistance to aminoglycosides, fluoroquinolones and macrolides could be due to selective antibiotic pressure. To our relief, none of our isolates were pandrug resistant.
Authors and Affiliations
Surinder Kumar, Diksha Budhani, Pallavi Sayal, Pooja Sindwani, Piyush Kumar Jain
Keywords
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- EP ID EP265509
- DOI 10.14260/Jemds/2017/1099
- Views 92
- Downloads 0
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