Endogenous PAF-acetylhydrolase affects the antigenicity of oxidized LDL<br /> in patients with coronary artery disease
Journal Title: Αρχεία Ελληνικής Ιατρικής - Year 2006, Vol 23, Issue 1
Abstract
OBJECTIVE The oxidative modification of LDL induces immunogenic epitopes, many of which are due to phospholipids formed during oxidation, such as oxidized phosphatidylcholine (oxPC), lysophosphatidylcholine (lyso-PC), platelet activating factor (PAF) and oxidized phospholipids. Lyso-PC is enzymatically generated during LDL oxidation through the hydrolysis of oxidized phospholipids by the LDL-associated PAF-acetylhydrolase (PAF-AH). The aim of this study was to evaluate the autoantibody titers against oxLDL in relation to the phase of LDL oxidation and to the activity of the LDL-associated PAF-AH in patients with coronary artery disease, in order to investigate a possible role of this enzyme in the immunogenicity of oxLDL. METHOD The autoantibody titers against various forms of oxLDL were estimated in 65 patients with stable angina pectoris and angiographically verified coronary artery disease (CAD) (7 women, 58 men, mean age 62.5±9.5 years) and in 47 healthy volunteers (control group). LDL isolated from fresh plasma was oxidized in the presence of 5 μΜ CuSO4. Three different forms of oxLDL were prepared, oxLDLL, oxLDLP and oxLDLD, i.e. at the end of the lag, propagation and decomposition phases, respectively. The same forms were also prepared after inactivation of endogenous PAF-AH [oxLDL(-)]. Malondialdehyde-modified LDL (MDA-LDL) was prepared and used as antigen. Autoantibody titers were measured by ELISA. RESULTS CAD patients had significantly higher titers than controls against oxLDLL (1.102±0.391 vs 0.873±0.254, P
Authors and Affiliations
E. LOURIDA, A. PAPATHANASIOU, J. GOUDEVENOS
Keywords
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