ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE T-786C POLYMORPHISM AMONG TYPE-2 DIABETES MELLITUS PATIENTS IN KHARTOUM, 2016
Journal Title: European Journal of Biomedical and Pharmaceutical Sciences - Year 2017, Vol 4, Issue 5
Abstract
Background: A single nucleotide polymorphism (T > C) rs2070744 due to transition of a thymine to a cytosine at T-786C in the promoter region of eNOS was found to reduce the rate of mRNA transcription by 50%, resulting in decreased serum NO levels which can inhibit apoptosis or stimulate tumor proliferation, angiogenesis and metastasis. Objective: To evaluate the association between Nitric Oxide Synthase gene T-786C polymorphism and type-2 Diabetes mellitus patients in Khartoum. Material and Methods: A total of 40 patients with type-2 Diabetes mellitus and50 control subjects were enrolled this study. DAN extrication samples previously extracted from EDTA Blood collected from SPV and store at- 20C. Analysis of the eNOS786T > C promoter polymorphism were carried out by allele specific polymerase chain reaction method (PCR). Results: The present study reported that, molecular analysis showed that, the most frequent genotype in patients was TT 100% (40 sample). While in the control group there was 100% TT genotype. Conclusion: The present study indicates the lack of association between eNOS and type-2 Diabetes mellitus which needs to be investigated in study with larger sample.
Authors and Affiliations
Ibrahim Khidir Ibrahim
Keywords
Related Articles
- EP ID EP628317
- DOI -
- Views 167
- Downloads 0
THE FAUNA AND ECOLOGY OF NEMATODES FROM THE FAMILY CYPRINIDAE, ORDER SPIRURIDA, IN ARTIFICIAL AND NATURAL BODIES OF WATER IN UZBEKISTAN
Fauna and ecology of nematodes of the order Spirurida of carp fishes of artificial and natural reservoirs of Uzbekistan F.D. Akramova, F.E. Safarova, D.A. Azimov, B.B. Soatov The modern species diversity of nematodes fro...
ASEAN – QUALITY REQUIREMENTS FOR GENERIC FILLING
ASEANrequires Process Validation report on three consecutive production batches which should include: Summary Introduction Batches used for validation Manufacturing equipment Critical process steps and parameters...
EPISTAXIS IN CHILDREN: COMPARITIVE ANALYSIS OF TOPICAL ANTISEPTIC AND CAUTERY VERSUS TOPICAL ANTISEPTIC ALONE
Objective: To evaluate and compare different modalities in management of simple recurrent epistaxis in children. Patients and methods: A total of 148 children (age 5-14 years) presented to ENT clinic with recurrent epist...
AWARENESS OF SMOKING-ASSOCIATED HEALTH RISKS AMONG MALE’S HIGH SCHOOLS STUDENTS IN WEST GAZA CITY
Background: Cigarette smoking is one of the main causes of preventable global death. More than 7 million people died as a result smoking and exposure to smokers. Smoking causes many serious cardiovascular and respiratory...
ANTIFUNGAL POTENTIAL OF QUERCUS INFECTORIA GALLS AGAINST CANDIDA ALBICANS-AN INVITRO STUDY
The promising potential of antimicrobial plant derived substances has attracted the attention of pharmaceutical and scientific communities during the last few years. The primary benefit of plant derived medicines is that...