Enhanced Plasma Persistence of Enzyme with Therapeutic Potential Using Polyethylene Glycol as a Coupling Agent
Journal Title: Tropical Journal of Natural Product Research - Year 2017, Vol 1, Issue 1
Abstract
Therapeutic proteins of microbial origin though possess good pharmacological activities, gets quickly broken down into amino acids by the action of proteases in the body. Hence to protect its activity and integrity the enzyme L-arginine deiminase (ADI) was coupled with a compatible polymer methoxypolyethylene glycol-succinimidyl succinate (mPEG-SS) and was further evaluated for the presence of its activity. Enzyme production was carried out by culturing Vibrio alginolyticus under optimal conditions and was purified by standard chromatographic techniques. The purified enzyme obtained was coupled with mPEG-SS 20k (1:50 mM) and purified. Coupling increased the molecular weight of the enzyme to about 112 kDa. The Km and Vmax values of immobilized enzyme were found to be 2.94 ± 0.13 mM and 129.87 ± 1.24 U/mL/min, respectively. Though there was a slight change in Km and Vmax values, it still retained its activity. Further, there was two-fold increase in the plasma half-lives of the enzymes. L-Arginine deiminase of Vibrio alginolyticus remained effective even after pegylation and showed enhanced plasma persistence in normal as well as tumour bearing mice. Hence it can be a promising candidate in treatment of L-arginine auxotrophic cancers. Further studies must be carried out to develop the enzyme in the form of chemotherapeutic agent.
Authors and Affiliations
Rahamat Unissa
Keywords
Related Articles
- EP ID EP349861
- DOI 10.26538/tjnpr/v1i3.5
- Views 115
- Downloads 0
Preliminary Investigation of the Possible Anti-inflammatory and Antioxidant Activities of Extract of Callichilia subsessilis Stapf. (Apocynaceae)
Received 06 December 2017 Revised 26 December 2017 Accepted 01 January 2018 Published online 07 January 2018 Callichilia subsessilis (Apocynaceae) is commonly found along the coast of West Africa. In South Eastern Nige...
Protective Effect of Ethanol Extract of Senna occidentalis Leaf Against Cadmium Induced Hepatotoxicity in Rats
Cadmium is a known environmental and industrial toxicant exposure to which leads to the generation of reactive oxygen species (ROS), with attendant oxidative damage to membrane lipids, cellular proteins and DNA. Recently...
Edible Mushrooms: As Functional Foods and Nutriceuticals
Mushrooms are macro-fungi that grow naturally on soil, agro wastes, on and around trees and are easily picked by hand. They occur in various sizes and shapes and may be edible or poisonous. Majority of mushrooms are ecto...
Anti-seizure activity of Extract of Jatropha gossypiifolia Linn (Euphorbiaceae)
Received 13 January 2018 Revised 27 January 2018 Accepted 02 February 2018 Published online 07 February 2018 The leaves of Jatropha gossypiifolia has been widely used for the treatment of epilepsy and childhood convuls...
Antiplasmodial and Acute Toxicity Studies of Fractions and Cassane-Type Diterpenoids from the Stem Bark of Caesalpinia pulcherrima (L.) Sw.
Received 08 March 2018 Revised 01 April 2018 Accepted 04 April 2018 Published online 07 April 2018 Malaria is a major public health disease affecting millions of people worldwide especially in subSaharan Africa, with a...