Enhanced Production of Amidotransferase From Bacillus Sp. ABP-6 By optimization of Nutritional Parameters Using Statistical Experimental Design
Journal Title: International Journal of Engineering and Science Invention - Year 2017, Vol 6, Issue 9
Abstract
The consortial effects of various nutritional parameters of medium on enhanced production of amidotransferase by Bacillus sp. APB-6 were studied using stepwise statistical experimental methodology. In addition to the effect of pH, temperature and inoculum size on enzyme production, a full factorial central composite design was chosen to explain nutritional constituents of medium viz. acetamide, glucose, yeast extract and sodium chloride for the analysis of the results. The Placket–Burman screening experiments suggested that sodium chloride, temperature, pH and n-methyl acetamide were the most influential media components. These four media components were optimized using a face centered design of response surface methodology (RSM) and the optimal combinations of the media constituents for enhanced amidohydrolase productions were determined as acetamide (3.0% (w/v), glucose 0.2% (w/v), yeast extract 1.5% (w/v), NaCl 0.35% (w/v), nonsubstrate inducer n-methyl acetamide 70 mM with inoculum 8% (v/v) at pH 7.0 and temperature 32.5oC. Thus in the present studies, 4.10 fold enhanced enzyme production (198.2 units/ mg dry cells) was achieved using response surface methodology.
Authors and Affiliations
Duni chand, Pankaj Kumari, Neena Devi
Keywords
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