ENHANCED PRODUCTION OF XYLANASE FROM WHITE ROT FUNGUS C. cinerea RM-1 NFCCI-3086BY STANDARDIZATION OF NUTRIENT SALT SOLUTION
Journal Title: International Journal of Agriculture Sciences - Year 2016, Vol 8, Issue 52
Abstract
One variable at a time approach has been employed to evaluate the effect of different components of NSS medium associated with xylanase production by the fungus C. cinerea RM-1 NFCCI 3086 using wheat bran and corn cob as substrate. Different sources of carbon, organic and inorganic nitrogen and surfactant were assessed in different concentrations for their significance on xylanase production under solid state fermentation. As a result of optimization, (NH4)2SO4 (3 g/L) was proved to be better in comparison to NH4Cl (4 g/L). Tween 80 (0.1 g/L) was also show good improvement in xylanase production. The xylanase activity was improved by 18.0% under optimized NSS medium (864.8 IU/mL) as compared to unmodified NSS medium (708.9 IU/mL).This standardized NSS medium shows very good enhancement in xylanase activity in a cost effective manner and can be used for further optimization studies of xylanase production.
Authors and Affiliations
POONAM MAAN
Keywords
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