ENTADA PHASEOLOIDES ATTENUATES SCOPOLAMINE INDUCED MEMORY IMPAIRMENT, NEURO-INFLAMMATION AND NEURO-DEGENERATION VIA BDNF/TRKB/NFΚB P65 PATHWAY IN RADIAL ARM MAZE
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 9
Abstract
Objective: The present study investigates the protective effects of crude extract from seeds of Entada phaseoloides and isolated compounds in enhancing cognition in Scopolamine induced learning and memory impairments in the radial arm maze model.Methods: Two doses (100 and 200 mg/kg) of the crude methanolic extract of Entada phaseoloides (MEEP) were evaluated for amnesic activity by Radial Arm Maze memory model. Scopolamine (0.4 mg/kg i. p.), an amnestic drug was used for impairing memory. Tacrine (3 mg/kg, i. p.) was used as the standard drug. Animals were sacrificed to evaluate biochemical parameters viz. lipid peroxidase (LPO), nitrite oxide (NO), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), cytokine levels by ELISA, Catecholamine levels by high performance liquid chromatography (HPLC), Caspase-3 activity by Fluorogenic assay, Gene (BDNF, TrkB, NF-κB p65, BAX) expression studies by Western Blotting and AChE, α7nAchR, Caspase-3, Nrf2 and HO-1 by Reverse Transcriptase Polymerase Chain Reaction.Results: Treatment with MEEP significantly decreases escape latency and reference memory error (RME). MEEP treatment reversed the Scopolamine-induced hyperactivation of Acetylcholinesterase activity and overexpression of proteins NF-κB p65 BAX and also Caspase-3 activity in the hippocampus of rats. The level of BDNF, TrkB and α7nAchR were significantly up-regulated and AChE, Caspase-3, Nrf2, HO-1 were down-regulated in the MEEP treated rat. The extract increased the activity of SOD, GSH, Catalase, 5-HT, NE, Dopamine and decreased the levels of LPO and NO in rat hippocampus. Different active components of the seeds have been isolated.Conclusion: These results indicated that seeds of Entada phaseoloides might become a promising therapeutic agent for the treatment of cognitive dysfunction in addition to its already established medicinal properties.
Authors and Affiliations
Chandana Choudhury Barua, Lipika Buragohain, Arundhati Purkayastha, Beenita Saikia, K Suresh Babu, G. Swarna Kumari, Acheenta Gohain Barua
Keywords
Related Articles
- EP ID EP566153
- DOI 10.22159/ijpps.2018v10i9.27487
- Views 59
- Downloads 0
GLUE ABUSE IN LOME: INVESTIGATION AND TOXICOLOGICAL STUDY ON WISTAR RATS
Objectives: Volatile substances abuse (VSA) is considered as one of the most dangerous forms of drug abuse leading to serious accidents and fatalities. This study aims to assess the extent and dangers of the sniffing of...
ANTI-DIABETIC ACTIVITY OF HYDROALCOHOLIC EXTRACT OF EUGENIA JAMBOLANA LEAVES IN ALLOXAN INDUCED DIABETIC RATS
Objective: The present study was carried out to evaluate the antidiabetic activity of hydroalcoholic extracts of Eugenia jambolana leaves. Methods: The hydroalcoholic extracts of Eugenia jambolana by alloxan induced diab...
EXPLORING VISCERAL ADIPOSITY INDEX AS A PREDICTOR OF VISCERAL ADIPOSITY DYSFUNCTION AND EVALUATING ITS PERFORMANCE IN PREDICTING HEPATIC INSULIN RESISTANCE IN INDIAN TYPE 2 DIABETICS
Objective: Visceral adiposity index (VAI) is a simple clinical algorithm developed as a surrogate marker for characterizing visceral adiposity dysfunction (VAD). This study aimed to explore an optimal VAI cut off value f...
IN VITRO AND OMICS TECHNOLOGIES OPENS A NEW AVENUE FOR DECIPHERING WITHANOLIDE METABOLISM IN WITHANIA SOMNIFERA
Withania somnifera, commonly known as Ashwagandha or winter cherry, is a principal medicinal plant that has been used in Ayurvedic and native medicine. In view of its varied therapeutic prospective, it has also been the...
NEUROTOX: A UNIQUE DATABASE FOR ANIMAL NEUROTOXINS
Objective: A wide range of toxic effects against other species are affected through potential protein molecules called neurotoxins. Among various kinds of toxins, neurotoxins play a critical role in life of other organis...