Enterotoxigenic Genes in strains of Staphylococcus spp., isolated from cheese made in Pamplona-Colombia
Journal Title: Revista MVZ Cordoba - Year 2015, Vol 20, Issue 1
Abstract
Objective. To determine the incidence of coagulase-positive strains of enterotoxigenic Staphylococcus in doble crema (double cream) cheese samples produced in Pamplona. Materials and methods. Bacterial isolation was performed following the routine method for coagulase positive Staphylococcus provided by the Colombian Technical Standard 4779, by using Baird Parker medium with confirmation of typical colonies by performing the coagulase test. Detection of genes for principal enterotoxins was done by PCR. Results. The prevalence of coagulase positive Staphylococcus in cheese samples was 31%, with 27% of the samples failing to meet the requirements of the NTC 750. In 24.6% of the studied isolates, genes for enterotoxin production were detected. The presence, in the isolated strains, of genes for SEB, SEA and SED was 18.5%, 4.6% and 3.0%, respectively. Conclusions. The significant presence of enterotoxigenic genes found in the isolates obtained from samples of double cream cheese made in Pamplona, suggests an important hazard to the health of consumers.
Authors and Affiliations
Fanny Herrera A, Ph. D *| Universidad de Pamplona, Facultad de Ciencias Básicas, Departamento de Microbiología, Grupo de Investigación en Microbiología y Biotecnología (GIMBIO). Ciudad Universitaria, Pamplona, Norte de Santander, Colombia.Correspondencia: fannyh@unipamplona.edu.co, Jesús Santos B, Ph. D| Universidad de León, Facultad de Veterinaria, Departamento de Higiene y Tecnología de los Alimentos. Campus de Vegazana, A.A. Nro. 24071, León, España.
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