Enumeration of Anaerobic Bacteria from Marketed Table Grape to Determine the Health Hazard in Urban Areas of Jessore, Bangladesh
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2016, Vol 11, Issue 2
Abstract
This study was conducted to identify contaminants in marketed white table grape for avoiding health hazard. White table grape samples were collected from Jessore, Bangladesh. The samples were inoculated into Nutrient Agar (NA) and Trypticase (Tryptic) Soy Agar (TSA) medium to obtain primary culture for bacteria. Following the inoculation the for bacterial colonies were found after incubation for 24 hours in 37⁰ C. Colony for bacteria were streaked into NA and TSA medium and incubated for 24 hours in 37⁰ C. Then pure cultures were identified by gram-staining technique, biochemical tests, carbohydrate fermentation tests and differential tests. Biochemical tests weredone byCatalase test, Oxidase test, Coagulase test, Urease activity test, Gelatin liquefication test, Citrate utilization test, Indole test, Methylene red test, and Voges-Proskauer test. Carbohydrate fermentation tests were included the utilization ofGlucose, Lactose, Sucrose, Maltose, L-rhamnose, L-arabinos, D-mannitol, Salinic, Glycerol, Cellbiose, Trehalose, Raffinose and Starch. Differential tests were initiated with Coagulase test, Novobiocin test and Hemolysis test. The morphologicalcharacteristics, cultural characteristics and results from different tests of isolated were compared with standard parameter and found to closely related to the genus Staphylococcus epidermidis.
Authors and Affiliations
Mizanur Rahman1, Sazin Islam, Md. Mahabub Hasan2, Md. Jahid Al Jakaria2, Mahmuda Sultana, Mohammad Rocky Khan Chowdhury
Keywords
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- EP ID EP383444
- DOI 10.9790/3008-1102018487
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