Epidemiology of Antimicrobial Resistance among Escherichia coli Strains in Trans-Nzoia County, Kenya
Journal Title: Journal of Microbiology and Infectious Diseases - Year 2016, Vol 6, Issue 3
Abstract
Objective: This study aimed to determine prevalence, antimicrobial susceptibility profile and the genetic basis to antimicrobial resistance, targeting blaTEM gene expression of diarrheagenic Escherichia coli among patients suffering from gastroenteritis in Kitale County Referral Hospital. Methods: A cross-sectional study design was adopted. A total of 103 fecal specimens were collected from participants ranging in age from two weeks to 82 years. E. coli was isolated and identified based on phenotypic and biochemical properties. Antimicrobial susceptibility was determined by Kirby-auer disk diffusion method. Polymerase chain reaction was used to detect the presence of blaTEM gene. Results: The prevalence of E. coli was 90.2% and age of the patient explained 53% of variation in prevalence. Isolates of diarrheagenic E. coli showed varied degree of susceptibility with sulfamethoxazole at 97%, co-trimoxazole 96%, ampicillin 84%, chloramphenicol 27%, tetracycline 16%, kanamycin 10% and streptomycin 9%. However, E. coli was highly sensitive to gentamicin at 96.8%. Approximately 42.2% of E. coli isolates were multidrug resistant to sulfamethoxazole, co-trimoxazole, ampicillin, chloramphenicol, tetracycline, kanamycin and streptomycin. All isolates that were resistant to ampicillin harbored blaTEM gene suggesting genetic mediation. Conclusion: The observed pattern of resistance to antibiotics points to the need to regulate their use and arrest buildup of resistant genes within the population. J Microbiol Infect Dis 2016;6(3): 107-112
Authors and Affiliations
Kebenei C. Kipkorir, Philip K. Bett, Patrick O. Onyango, Cecilia M. Onyango, Cyrus Ayieko, Paul O. Ang’ienda
Epidemiology of Hepatitis C Virus and Human Immunodeficiency Virus Co-Infection and Challenges in Their Diagnosis
Objective: To screen HCV infection in HIV infected individuals and to compare the genotype and viral load of HCV in these co infected patients versus HIV negative individuals. Methods: Serum samples from 70 HIV infected...
Ethionamide depolama sıcaklığının duyarlılık testine etkisi
Amaç: Ethionamide ısıya duyarlı yapısı nedeniyle, ilaç duyarlılık testinde (DST) ısının etkisini ve sıvı veya katı ortamdaki dayanıklılık durumunun araştırılması amaçlanmıştır. Yöntemler: Ethionamide çalışma solüsyonu v...
Kronik Hepatit C’li hastalarda pegylated ınterferon/ribavirin ikili tedavisi: 323 hastanın sonuçları
Amaç: Pegile interferon-α (PEG-IFN) ve ribavirin kombinasyonu Kronik Hepatit C (KHC) tedavisinde standarttır. Bu tedavinin yeni yaklaşımlar eşliğinde KHC’li hastalarda etkinliğinin araştırılması amaçlandı. Yöntemler: Or...
Brusella spondilodiskiti: Torasik ve lomber bölgenin multifokal tutulumu; nadir bir olgu
Brusellozis bir çok organ ve dokuları tutabilen bir zoonotik enfeksiyondur. İskelet sistemi bruselloziste en sık etkilenen bölge olup, sıklığı % 2 ile % 53 arasında değişmektedir. Multifokal spinal tutulum nadirdir....
Antibiotic Therapy for ESBL and NDM producing Escherichia coli and Klebsiella pneumoniae isolates in a Tertiary Care Center
Antibiotic Therapy for ESBL and NDM producing Escherichia coli and Klebsiella pneumoniae isolates in a Tertiary Care Center Vedant Vikrom Borah1,2, Mrinal Kumar Baishya2, Kandarpa Kr Saikia2 1Department of Microbiology,...