ESBL-Producing Escherichia coli Isolated from Children with Acute Diarrhea – Antimicrobial Susceptibility, Adherence Patterns and Phylogenetic Background
Journal Title: Advances in Clinical and Experimental Medicine - Year 2012, Vol 21, Issue 2
Abstract
[b]Background. [/b]Escherichia coli remains the principal bacterial pathogen in childhood diarrhea and constitutes an important public health problem, especially in developing countries. Diarrheagenic E. coli strains often display resistance to β-lactams due to the production of extended-spectrum β-lactamases (ESBLs). [b]Objectives.[/b] A total of thirty ESBL-producing E. coli strains colonizing the gastrointestinal tracts of children with acute diarrhea were studied in order to determine their antimicrobial susceptibility, adherence patterns to the HEp-2 cell line and phylogenetic background. [b]Material and Methods.[/b] ESBL production was detected by the double disk synergy test (DDST). The minimal inhibitory concentrations (MICs) of antibacterial drugs were determined by an agar dilution technique on Mueller-Hinton agar. The presence of blaTEM, blaSHV and blaCTX-M determinants in the strains studied was ascertained by polymerase chain reaction (PCR). [b]Results.[/b] The strains displayed the resistance pattern typical of ESBL producers. The majority of them (23 out of 30) were found to produce CTX-M-type ESBLs conferring a high level of resistance to oxyimino-β-lactams, especially to cefotaxime and ceftriaxone. In many cases, the strains exhibited resistance to non-β-lactam antimicrobials, such as gentamicin, amikacin, co-trimoxazole and tetracycline. On the other hand, these strains were uniformly susceptible to carbapenems, to oxyimino-β-lactams combined with clavulanic acid and to tigecycline. The E. coli strains were distributed among the four main phylogenetic groups: A, B1, B2 and D. The in vitro adhesion assay revealed that all but two of the strains adhered to the HEp-2 epithelial cell line. Aggregative and diffuse adherence patterns were found to be the most prevalent. [b]Conclusions.[/b] CTX-M-type enzymes were the most prevalent ESBLs among the strains studied. As many as 40% of the diarrheagenic E. coli isolates were found to belong to phylogenetic group D, which usually comprises E. coli strains associated with extraintestinal infections. The effectiveness of tigecycline against ESBL-producing E. coli strains was similar to that of imipenem and meropenem.
Authors and Affiliations
Roman Franiczek, Beata Sobieszczańska, Michał Turniak, Urszula Kasprzykowska, Barbara Krzyżanowska, Katarzyna Jermakow, Grażyna Mokracka-Latajka
Keywords
Related Articles
- EP ID EP103406
- DOI -
- Views 75
- Downloads 0
Stężenie aldehydu dimalonowego, mieloperoksydazy i nitrotyrozyny u chorych na przewlekłe wirusowe zapalenie wątroby B i C
[b]Wprowadzenie.[/b] Stres oksydacyjny jest jednym z potencjalnych mechanizmów biochemicznych w patogenezie przewlekłego wirusowego zapalenia wątroby. [b]Cel pracy.[/b] Określenie poziomu stresu oksydacyjnego w dużej gr...
Nietrzymanie moczu u kobiet i jego związek z ciążą, rodzajem porodu, chorobą tkanki łącznej i innymi czynnikami
[b]Cel pracy.[/b] Określenie związków między nietrzymaniem moczu (n.m.) a wskaźnikami, takimi jak ciąża, rodzaj porodu i inne. [b]Materiał i metody.[/b] Badanie zostało oparte na kwestionariuszu, który wypełniło 761 pac...
Clinical and Biochemical Factors Affecting Postoperative Hypocalcemia After Near-Total Thyroidectomy
<strong>Background. </strong>The most common complication which occurs after thyroidectomy is postoperative hypocalcemia due to parathyroid gland damage. It usually appears 24–48 hours postoperatively, but there ar...
Stężenie IL-15 i IL-18 w płynie owodniowym w drugim trymestrze ciąży u płodów z prawidłowym kariotypem i aberracjami chromosomowymi
[b]Wprowadzenie.[/b] Niewiele wiadomo o zachowaniu się interleukiny 15 (IL-15) i 18 (IL-18) w płynie owodniowym w II trymestrze ciąż powikłanych aberracjami chromosomowymi u płodu. Do tej pory nie rozstrzygnięto także, c...
Urinary Incontinence in Women and Its Relation with Pregnancy, Mode of Delivery, Connective Tissue Disease and Other Factors
[b]Objectives.[/b] The aim of this study was to determine the relationship between urinary incontinence (UI) and parameters such as pregnancy, mode of delivery and other factors. [b]Material and Methods.[/b] The study w...