Establishing monoxenic culture of arbuscular mycorrhizal fungus Glomus intraradices through root organ culture
Journal Title: Journal of Applied and Natural Science - Year 2014, Vol 6, Issue 1
Abstract
Arbuscular mycorrhizal fungi are soil fungi distributed worldwide, forming symbiosis with most of the vascular plants for their growth and survival, which is used for sustainable agriculture and ecosystem management. This study investigated the establishment of monoxenic cultures of Glomus intraradices in association with transformed carrot hairy root. The G.intraradices spores were isolated from sugarcane rhizosphere by wet sieving and decanting technique and propagated in open pot culture. Transformation in to carrot hairy root was done using Agrobacterium rhizogenes. Surface sterilization of G.intraradices spores co-cultured with transformed carrot hairy root in Modified Strulla and Romand (MSR) medium was found the host root growth as well as for germination AM spores. After three months of incubation in dark condition, significant production of extensive hyphal growth on MSR medium and an average of 8500-9000 spores per petri dish was observed. The in vitro inoculum exhibited higher potential of root colonization due to numerous intraradices mycelium with extensive spore load. The produced monoxenic inoculum can be used in place of traditional system where it has a advantage of producing contaminant free propagulas. Thus the monoxenic culture system, a powerful tool, of AM sporulation, can be used for the mass production of monoxenic inoculum of AM fungi besides studying its biology.
Authors and Affiliations
M. Srinivasan, K. Kumar, P. Marimuthu
Keywords
Related Articles
- EP ID EP131777
- DOI 10.31018/jans.v6i1.417
- Views 125
- Downloads 0
Influence of operating conditions on the electrolytic treatment for the removal of color, TSS, hardness and alkalinity using Al-Al electrode combination
The present investigation observed the effect of current density (CD), operating time (OT), inter electrode distance (IED), electrode area (EA), initial pH and settling time (ST) using Al-Al electrode combination on the...
Phenology and thermal indices of maize (Zea mays L.) influenced by subsoil compaction and nitrogen fertilization under semi-arid irrigated conditions
The magnitude of yield reduction due to soil compaction is variable and depends on the soil type, fertility status and other soil and environmental factors. The present investigation was carried out at the research farm,...
Effect of plant spacing and fertility level on leaf area variation at different phenological stages of cape gooseberry (Physalisperuviana L.) grown in sodic soil
Vegetative and reproductive growth in cape gooseberry (Physalisperuviana L.) proceed concomitantly during the greater part of the life cycle thereby foliar traits (e.g. leaf area) become important in photosynthetic actio...
Bio-rational insecticides toxicity against Liriomyza trifolii (Burgess) damaging Cantaloupes, Cucumis melo var. cantalupensis
Field experiments were conducted for two seasons (2015-16 and 2016-17) to evaluate bio-rational insecticides against leaf miner (Liriomyza trifolii) damaging cantaloupes and it was found that among various insecticides v...
Taxonomic studies of Eulophid parasitoids (Hymenoptera: Chalcidoidea) collected from Uttarakhand, India
A sound taxonomic knowledge base is a prerequisite for effective conservation, environment assessment, ecological research, management and sustainable use of biological resources. Parasitoids are the major component of t...