ESTABLISHMENT AND APPLICATION OF A QPCR-BASED METHOD FOR WOLBACHIA GENOTYPE AND DENSITY DETECTION IN AEDES ALBOPICTUS
Journal Title: Acta Parasitologica et Medica Entomologica Sinica - Year 2024, Vol 31, Issue 1
Abstract
[Objective] Naturally, Aedes albopictus carries both the A and B supergroups of Wolbachia strains, which are symbiotic bacteria that can influence the transmission of mosquito-borne diseases by inhibiting the ability of their host to transmit viruses or by reducing the population density of their host, and the strength of this effect is closely related to its density in host cells. In this study, an accurate qPCR-based method for detecting Wolbachia genotype and density in mosquitoes was established. [Methods] The wsp gene of Wolbachia carried by Ae. albopictus was cloned and sequenced, and specific primers for the non-conserved regions of the wAlbA and wAlbB wsp genes were designed. [Results] The application of this method showed great sensitivity and specificity. The primers did not cross-react between the two Wolbachia supergroups. Further, with this method, it was possible to detect decreases in Wolbachia density in host cells following treatment with tetracycline at different concentrations, and the optimal tetracycline treatment concentration was determined to be 0.1 mg/mL. [Conclusion] This method can provide technical supports for studies of the effects of Wolbachia on physiological reproduction and innate immunity in mosquitoes.
Authors and Affiliations
Yi-ping XIONG, Ning-xin ZHOU, Guo-rui LIANG, Jian-hang LI, Bo LI, Wan-rong HAN, Dan XING, Xiao-xia GUO, Yu-ting JIANG, Tong-yan ZHAO
Keywords
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- EP ID EP753968
- DOI 10-3969/j.issn.1005-0507-2024-01-004
- Views 22
- Downloads 0
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