ESTIMATION OF CELECOXIB IN HUMAN PLASMA BY RAPID AND SELECTIVE LC-MS/MS METHOD FOR A BIOEQUIVALENCE STUDY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 10
Abstract
Objective: A selective, sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay has been developed for the determination of celecoxib (CXB) in negative ionization mode.Methods: Celecoxib and celecoxib-D7 (CXB-D7) as internal standard (IS) were extracted from 300 µl human plasma by solid-phase extraction using strata-X SPE cartridges. Chromatographic separation was achieved on ACE C8-300 (50 × 4.0 mm, 3.0 μm) column using methanol-1.0 mmol ammonium acetate solution in 80:20 (v/v) ratio. The protonated precursor to product ion transitions studied for CXB and CXB-D7 were m/z 380.0 → 315.9 and 387.0 → 323.0, respectively.Results: The limit of detection (LOD) and lower limit of quantitation of the method were 2.50 and 10.0 ng/ml respectively with a linear dynamic range of 10.0-4000 ng/ml for CXB. The intra-batch and inter-batch precision (% CV) and mean relative recovery across quality control levels is<7.2 % and 85.5 % respectively. Matrix effect in human plasma, expressed as IS-normalized matrix factor ranged from 0.99-1.03.Conclusion: The method was successfully applied in healthy subjects using a single dose of 400 mg celecoxib capsules under fasting and fed conditions. The reproducibility in the measurement of study data is demonstrated by incurred sample reanalysis.
Authors and Affiliations
Nirav P. Patel, Mallika Sanyal, Pranav S. Shrivastav, Bhavin N. Patel
Keywords
Related Articles
- EP ID EP566122
- DOI 10.22159/ijpps.2018v10i10.28289
- Views 177
- Downloads 0
THE ROLE OF ETHYL ACETATE FRACTION OF ANDROGRAPHIS PANICULATA AND DOXORUBICIN COMBINATION TOWARD THE INCREASE OF APOPTOSIS AND DECREASE OF VEGF PROTEIN EXPRESSION OF MICE FIBROSARCOMA CELLS
Objective: The objective of this study is to prove the role of an ethyl acetate fraction of Andrographis paniculata and doxorubicin combination toward the increase of apoptosis and decrease of VEGF protein expression of...
METFORMIN IN THE PREVENTION OF METABOLIC SYNDROME ASSOCIATED WITH INITIATION OF ATYPICAL ANTIPSYCHOTIC THERAPY IN ADOLESCENTS AND YOUNG ADULTS - A RANDOMIZED, OPEN LABELLED, SINGLE CENTERED STUDY
Objective: The aim of the study was to evaluate the effectiveness and safety of Metformin along with Risperidone to prevent antipsychotic-induced metabolic syndrome in first-episode schizophrenia patients.Methods: This w...
CYTOTOXIC EFFECT OF HELIOTROPIUM INDICUM EXTRACTS ON HELA CELL LINE
Objective: Heliotropium indicum (H. indicum) has been used widely for centuries on warts and to treat inflammations and tumors. H. indicum Linn (Family Boraginaceae) is a medicinal plant. It is known commonly as “Cockâ...
IDENTIFICATION OF PUTATIVE DRUG TARGETS IN MASTITIS CAUSING STAPHYLOCOCCUS AUREUS BY IN SILICO APPROACH
Objective: In the present study an attempt has been made by the use of a computational approach to investigate putative drug targets in Staphylococcus aureus.Methods: In silico comparative analysis of the metabolic pathw...
MICROENCAPSULATION OF PULP OF MANGIFERA INDICA L. BY SPRAY DRYING AND ANTIOXIDANT ACTIVITY
Introduction: For the food industry and trade, it is very important to obtain powders based on fruits or vegetables by means of drying techniques that allow them to preserve their nutritional, organoleptic properties and...