Estimation of Lipid Accumulation in Chlorella pyrenoidosa culturing in Different Concentrations of KNO3
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2016, Vol 4, Issue 10
Abstract
The present study deals with growing microalgae Chlorella pyrenoidosa to assess its biofuel potential. The microalga was grown in Fogg’s media with different concentration of nitrate source. And an attempt has been made to increase lipid content in algae in nitrogen stress condition. Chlorella pyrenoidosa was isolated from sewage water by step dilution method. Different concentration of KNO3 (0-0.4g/L) was used in Fogg’s media. Eventually biomass production was decreased with decreasing concentration of nitrate in media but lipid content increased. The result showed that lipid accumulation is higher in stationary phase than in exponential phase at same concentration of nitrate. In culture media with 0.05g/L of KNO3 maximum lipid content of 28% was recorded. The experimental study concluded that nitrogen starvation is a promising method to enhance lipid productivity in microalgae and it can be a big tool in biofuel production project.
Authors and Affiliations
Ramandeep Kaur, Anupama Mahajan, Anjana Bhatia
Keywords
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