Estimation of the Degree of Diversity for Some Iraqi Wheat Varieties through ISSR, SRAP and RAPD Markers

Journal Title: Journal of Experimental Agriculture International - Year 2016, Vol 11, Issue 3

Abstract

DNA-based molecular markers such as Inter Simple Sequence Repeat (ISSR), Sequence-Related Amplified polymorphism (SRAP) and Random Amplified Polymorphic DNA (RAPD) were used in this study to examine the genetic differences among sixteen Iraqi wheat varieties. Seventy three primers out of 177 were reproducible and showed clear amplified bands. The degree of genetic diversity, Polymorphism information content (PIC) and resolving power (RP) were estimated. All the studied molecular markers were informative and showed good ability to classify and distinguish 16 wheat varieties. Total number of polymorphic bands is 134, 221 and 55 for ISSR, SRAP and RAPD respectively. PIC and RP values were 0.259, 0.264 and 0.262 and 9.06, 7.87 and 2.78 respectively. It seems that SRAP marker was the most optimal technique for characterization the of Iraqi wheat varieties. The most efficient primers were Ne ISSR 7 for ISSR; Me4-Em3 and Me4-Em9 primers combination for SRAP; Operon primers K14, K15 and K19 for RAPD. The results of this study can be used to as starting point of the data base for Iraqi wheat varieties to be used in the future development of new varieties in a breeding programs.

Authors and Affiliations

Dhafir H. Al-Kaab, Majid Sh. Hamdalla, Ismail Dweikat, Noora J. Al-Saedi

Keywords

Related Articles

Increase of Spinach Growth Through the use of Larger Plug Cell Volume and an Exogenous BAP Spray

Aims: In spinach, transplanting has replaced direct seeding but small containers may cause root restriction effects that would be decreased post-transplant yield. Although a single hormonal regulator (BAP) spray is effec...

Plant Species Characteristics and Woody Plant Community Types within the Historical Range of Savannah Elephant, Loxodonta africana Blumenbach 1797 in Northern Togo (West Africa)

Aims: Assessment of plant species diversity and habitat typology. Study Design: Stratified random sampling design according to defined land categories of a land cover map. Place and Duration of Study: Complex of protecte...

Perception of Youth Roles in Agricultural Innovation Management System among Arable Crop Farmers in Farming Communities of Osun State, Nigeria

This paper investigated the arable crop farmers’ perception of youth roles in agricultural innovation management system in farming communities of Osun State. Specifically, problems militating against the effective partic...

Combining Ability of Yield Related Traits and Gene Interaction on Tomato (Lycopersicon escullentum Mill.) in Yola

Field experiments were conducted to estimate the combining ability and nature of gene action governing inheritance of characters in tomato (Lycopersicon escullentum Mill) under high temperature stress. The experiment was...

Influence of Human Urine-compost Mixture on Some Soil Fertility Properties, Yield and Shelf-life of Tomato (Solanum lycopersicon)

Human urine, especially if combined with composts, could be a good source of fertilizer for improving soil fertility and ultimately, crop production. Thus, the focus of this study was to evaluate the influence of human u...

Download PDF file
  • EP ID EP351320
  • DOI 10.9734/AJEA/2016/20371
  • Views 104
  • Downloads 0

How To Cite

Dhafir H. Al-Kaab, Majid Sh. Hamdalla, Ismail Dweikat, Noora J. Al-Saedi (2016). Estimation of the Degree of Diversity for Some Iraqi Wheat Varieties through ISSR, SRAP and RAPD Markers. Journal of Experimental Agriculture International, 11(3), 1-11. https://europub.co.uk/articles/-A-351320