ESTROGENIC AND ANTIESTROGENIC ACTIVITIES OF COMMERCIAL DIETARY SUPPLEMENTS CONTAINING HERBAL INGREDIENTS AND ISOFLAVONES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2016, Vol 8, Issue 11
Abstract
Objective: This study aimed to assess the estrogenic and antiestrogenic activities of five dietary supplements, commercially available for treatment of menopausal symptoms, before and after gastrointestinal digestion by employing a yeast steroid-regulated transcription system.Methods: Supplements (S) were extracted with either 80% methanol or water. Water extracts were subjected to simulated gastrointestinal (GI) digestion. Estrogenic and antiestrogenic activities were assessed by a steroid-regulated transcription system in Saccharomyces cerevisiae expressing the human estrogen receptor alpha.Results: The highest estrogenic activities were detected in both S1 methanol (2342.5±20.83 MU) and water (1225.6±20.6 MU) extracts (400 estradiol equivalents). Extracts showed antiestrogenic properties by reducing the transcriptional activity induced by estradiol in transgenic yeast. The highest antiestrogenic activity was detected in S2 methanol extract and S3 water extract, which inhibited estradiol activity by 76% and 64%, respectively. After GI digestion, S1, S2 and S3 extracts showed significantly higher estrogenic and antiestrogenic activities as ‘serum-available’ than ‘colon-available’ samples and S4 and S5 extracts showed significantly higher activities as ‘colon-available‘ than ‘serum-available‘ samples.Conclusion: All dietary supplements revealed estrogenic and antiestrogenic activities. The GI digestion demonstrated the availability of phytoestrogens for absorption in the blood stream. Supplements containing soy isoflavones and alfalfa ingredients had the highest estrogenic activities and could be more effective than supplements with complex plant formulation in alleviating menopausal symptoms and treating osteoporosis. The transgenic yeast assays proved to be a powerful tool for assessing the in vitro estrogenic and antiestrogenic activities of dietary supplements.Â
Authors and Affiliations
Paramita Basu, Sharon Sunny, Camelia Maier
Keywords
Related Articles
- EP ID EP575926
- DOI 10.22159/ijpps.2016v8i11.14687
- Views 52
- Downloads 0
KINETIC MODELING OF RASAGILINE MESYLATE FROM NANOSCALE SOLID LIPID PARTICLES
Objective: The present study is aimed to investigate the In-vitro release behavior of the Rasagiline mesylate loaded nanoscale solid lipid particles to provide the quality based controlled release system. Methods: The Ra...
EFFECT OF AMLODIPINE AND ENALAPRIL ON WOUND HEALING IN DIABETIC WISTAR ALBINO RATS
Objective: A number of structural and functional mechanisms have been identified in the pathogenesis of impaired wound healing in diabetes. Diabetes promotes endothelial dysfunction as evidenced by decreased nitric oxide...
ACUTE DERMAL TOXICITY OF COAL FLY ASH NANOPARTICLES IN VIVO
Objectives: To study the toxicity effects of coal fly ash nanoparticles-induced acute dermal toxicity in Wistar albino rats.Methods: Acute dermal toxicity studies for coal fly ash nanoparticles (CFA-NPs) were conducted i...
SAMBUCUS NIGRA LINN: IN VIVO STUDIES ON RABBITS
Objective: The research work was carried out to explore the safety and efficacy of a low dose of Sambucus nigra Linn [S. nigra] extract on hematological, biochemical and histopathological parameters of rabbits organs (ki...
A RAPID STABILITY INDICATING HPLC-METHOD FOR DETERMINATION OF NORETHISTERONE ACETATE IN PLASMA, TABLETS AND IN A MIXTURE WITH OTHER STEROIDS
Objectives: This study reports about a simple, rapid and accurate, routine-HPLC method for quantification of Norethisterone acetate (NA) in bulk powder, tablets and plasma.Methods: The chromatographic separation is carri...