ETHANOL EXTRACT AND ACTIVE FRACTION EFFECT OF Moringa Oleifera. Lam IN INHIBITING COX-2 ACTIVITY ON MCF-7 CELL
Journal Title: Asian Journal Of Pharmaceutical Research and Development - Year 2018, Vol 6, Issue 4
Abstract
The expression of COX-2 is involved in carcinogenesis through the proliferative process, angiogenesis and inhibition of apoptosis. Moringa oleiferahas been shown to function as an anti-oxidant, anti-inflammatory, anti-microbial and anticancer. Moringa leaf extraction was done by percolation method, and continued to liquid-liquid extraction to obtain nonpolar, semipolar, and polar fractions using n-hexane and ethylacetate solvent.The aim of this study was to determine the effect of ethanol extract and the fraction of n-Hexane of Moringa oleiferaleaf in decreased MCF-7 breast cancer cell and COX-2 expression. This study used true experimentation in vitro design using MCF-7 cells. MTT Assay was performed using a therapeutic dose 6,125-1000 μg / ml to determine IC50 in MCF-7 cells. IC50 of ethanol extract was obtained 94.44 μg / ml and 97.60 μg / ml for n-Hexane fraction. Selectivity index of both samples was 3.95 μg / ml and 2.87 μg / ml. The expression of COX-2 was tested qualitatively with immunocytochemical methods and showed that ethanol extract and n-hexane fraction of Moringa oleiferaleaf were able to suppress COX-2 enzyme expression on MCF-7 cells depending on concentration
Authors and Affiliations
D Azizah
ADVANCEMENT IN TRANSDERMAL DRUG DELIVERY SYSTEM : MICRONEEDLES
One of the thrust areas in drug delivery research is transdermal drug delivery systems (TDDS) due to their characteristic advantages over oral and parenteral drug delivery systems. Researchers have focused their attentio...
3D PRINTING OF PHARMACEUTICALS –A POTENTIAL TECHNOLOGY IN DEVELOPING PERSONALIZED MEDICINE
The 3D PRINTING technology has caught the attention of medical devices industry and pharmaceutical industry due to its applications on various platform in health care industry. Even though this tech...
ETHANOL EXTRACT AND ACTIVE FRACTION EFFECT OF Moringa Oleifera. Lam IN INHIBITING COX-2 ACTIVITY ON MCF-7 CELL
The expression of COX-2 is involved in carcinogenesis through the proliferative process, angiogenesis and inhibition of apoptosis. Moringa oleiferahas been shown to function as an anti-oxidant, anti-inflammatory...
ANALYSIS OF PATENT TRENDS BETWEEN 2000 AND 2017 IN THE AREASOF MEDICAL, DENTAL AND TOILET PREPARATIONS INCLUDING OF PHARMACEUTICALS
Patent information is highly important for decision makers. Their demand for latest technology trend and competitor analysis poses new challenges with respect to the processing and visualization of large amount of patent...
PREGNANCY: AN OVERVIEW OF C-SECTION DELIVERY
Cesarean section (CS) is a surgical procedure used to deliver one or more babies. CS is usually performed when vaginal delivery will put the mother or child’s health or life at risk. Since CS also involves risk for adver...