ETHNOPHARMACOLOGICAL EVALUATION OF MEDICINAL PLANTS FOR CYTOTOXICITY AGAINST VARIOUS CANCER CELL LINES
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2017, Vol 9, Issue 5
Abstract
Objective: To evaluate the cytotoxic potential of leaves and seeds of Hibiscus sabdariffa L., fruit juice of Phyllanthus emblica, rhizomes of Dryopteris cochleata and flowers of Caesalpinia decapetala (Roth) Alston along with the chemical profiling of the most toxic extract through Gas-mass spectroscopy-MS technique.Methods: The hydroalcoholic extract of the selected crude drugs was prepared by maceration method and the extracts were undergone through phytochemical analysis. The cytotoxic activity of the hydroalcoholic extract was performed against four cancer cell lines i.e. liver (HepG2), breast (MCF7), prostate (PC-3) and leukemia (HL60) using sulphorhodamine B assay. The hydroalcoholic extract of Caesalpinia decapetala flowers was profiled through using gas mass spectroscopy.Results: The results confirmed that Phyllanthus emblica inhibited HL60 cancer cells at the dose of 35.6 µg/ml and show dose-dependent growth inhibition. The flowers of Caesalpinia decapetala inhibited nearly fifty percent of HL60 cancer cells at very low dose i. e 10 µg/ml. The analysis of Caesalpinia decapetala flowers shows the presence of diterpenoid furanolactones, bufadienolides, polycyclic enones, and androsterone.Conclusion: The fruit juice of Phyllanthus emblica and flowers of Caesalpinia decapetala showed good inhibitory activity against HL60 cancer cell line. The use of Phyllanthus emblica in herbal medicine is justified. The data obtained impelled to further assess the in vivo efficacy of Caesalpinia decapetala flowers for anticancer activity.
Authors and Affiliations
Ruchi Singh Thakur, Bharti Ahirwar
Keywords
Related Articles
- EP ID EP575655
- DOI 10.22159/ijpps.2017v9i5.17289
- Views 77
- Downloads 0
TO STUDY THE ROLE OF EXERCISE AND DIETARY MANIPULATION IN OBESE RATS TREATED WITH ORLISTAT
Objective: The present study was undertaken to evaluate the role of exercise and dietary manipulation in obese rats treated with Orlistat.Methods: The study was conducted for 16 weeks. High-fat diet (HFD) was used to ind...
MINING SINGLE NUCLEOTIDE POLYMORPHISM FROM PUBLICLY AVAILABLE ESTS OF BREAD WHEAT (TRITICUM AESTIVUM L.)
Objective: The present study was undertaken to discover Single Nucleotide Polymorphisms (SNPs) in bread wheat with reference to leaf rust disease.Methods: Next Generation Sequencing platform sequencing by Oligonucleotide...
IN-VITRO COMPARATIVE STUDY OF ANTI-INFLAMMATORY AND ANTI-ARTHRITIC EFFECTS OF FLEMINGIA STRICTA ROXB AND NYMPHAEA NOUCHALI LEAF
Objective: To evaluate the comparative study of anti-inflammatory, anti-arthritic activity of methanol extract of Flemingia stricta and Nymphaea nouchali leaf.Methods: Human Red Blood Cell (HRBC) membrane stabilization m...
AZATHIOPRINE INDUCED PANCYTOPENIA-A CASE REPORT
Azathioprine (AZA), a prodrug of 6 mercaptopurine, is an immunosuppressant that can be used as adjunctive therapy with corticosteroids in the treatment of arteritic form of ischemic optic neuropathy. Although myelotoxici...
THE EFFECTS OF DIABETES MELLITUS ON THE RESPONSE TO PEGINTERFERON-ALPHA IN COMBINATION WITH RIBAVIRIN THERAPY IN EGYPTIAN CHRONIC HEPATITIS C PATIENTS
Objective: The present study aimed to determine whether type 2 diabetes mellitus influences the response to antiviral therapy with peg-interferon alpha plus ribavirin in Egyptian patients with chronic hepatitis C.Methods...