Evaluating the Correlation of Different Culture Media for the Identification and Isolation of Common Gram Positive Uropathogens and Yeast Causing UTI
Journal Title: International Journal of Advanced Microbiology and Health Research (IJAMHR) - Year 2018, Vol 2, Issue 3
Abstract
Background: Urinary tract infection (UTI) is a common problem diagnosed and treated in urgent care medicine practice. Urinary tract infection (UTI) is a collective term that describes any infection involving any part of the urinary tract, namely the kidneys, ureters, bladder and urethra. Clinicians need to be aware of the advantages and limitations of diagnostic tests. Urine cultures occupy most of the workload of routine microbiology laboratories in developing country . Accurate and rapid identification of pathogens is important for a clinical microbiology laboratory. Materials and Methods: Mid-stream urine and catheterized samples were collected. Cultures were plated on blood agar, MacConkey agar and cysteine lactose electrolyte deficient media and incubated overnight at 35°C-37°C in ambient air. Colonies on the MacConkey agar, CLED agar and blood agar were also identified. The final identification of the isolates was done using standard identification protocol. Results: Out of 500 urine samples processed, 199 showed pure growth .Out of 199 pure growths, 56 were gram positive cocci and 17 were yeast. Out of all gram positive cocci showed growth but most of the gram-positive cocci and yeast were unable to grow on Mac-Conkey agar and blood agar but grew successfully on CLED agar. Conclusions: So, in resource constrain laboratories, CLED agar can be used as media of choice for isolation of common uropathogens , cost effective and decreases work load of the laboratories.
Authors and Affiliations
Aarti Chaturvedi
Keywords
Related Articles
- EP ID EP391745
- DOI -
- Views 79
- Downloads 0
Seroprevalence of Mycoplasma gallisepticum in Broiler Chicken by ELISA
Background: This study aimed to determine the sero-prevalence of Mycoplasma gallisepticum (MG) which causes chronic respiratory disease in poultry. It causes huge economic loss to the poultry industry. Materials and Meth...
Perception of Suicide among Higher Secondary Level Students
Background: A descriptive study entitled "Perception of Suicide among Higher Secondary Level Students" was carried out to find out the perception of suicide among 130 students of New Life Higher Secondary School, Bardagh...
Evaluating the Correlation of Different Culture Media for the Identification and Isolation of Common Gram Positive Uropathogens and Yeast Causing UTI
Background: Urinary tract infection (UTI) is a common problem diagnosed and treated in urgent care medicine practice. Urinary tract infection (UTI) is a collective term that describes any infection involving any part of...
Perception of Clinical Learning Environment among Nursing Students
Background: The descriptive study entitled Perception of Clinical Learning Environment (CLE) among Nursing Students was carried out to find the perception of clinical learning environment among nursing students with the...
Comparison of Phenotypic Methods for Detection of Metallo-Beta- Lactamases in Gram Negative Bacteria at a Tertiary Care Hospital
Metallo-β-lactamase (MBL)-producing bacteria lead to resistance to carbapenem an antibiotic that is used as the last resort for treatment of multidrug-resistant bacteria, extended spectrum beta-lactamases, and AmpC β-lac...