Evaluating the effect of genistein on the amount of BDNF protein in the spinal cord of diabetic ovariectomized rats
Journal Title: International Journal of Medical Research & Health Sciences - Year 2016, Vol 5, Issue 4
Abstract
BDNF (Brain derived neurotrophic factor) is a secretion protein and a member of the neurotrophin family of growth factors. Studies have shown that in ovariectomized animals, the expression of BDNF decreased. However, recommending more estrogen increases its expression. Genistein is a phytoestrogen found in soy and soy products in large amount. The compound has attracted medical science researchers and public individuals, owing to the fact that it helps in the prevention and treatment of several diseases such as diabetes, menopause symbols, osteoporosis, heart and blood vessel diseases, renal disease, and different cancers. In this study, the effect of genistein was studied as an alternative to estrogen in the expression of BDNF in ovariectomized rats of type 2diabetes. Sixty (60) female rats were randomly divided into 4 groups. The SHAM group received laparotomy and drug solution (DMSO+PEG). In the ovariectomy group (OVX), the ovary on both sides of the abdomen were removed. The diabetic ovariectomy group (OVX. DI) had type 2 diabetes after ovariectomy. In the diabetic ovariectomy group treated with genistein (OVX. DI. G), the rats received genistein 1 mg / kg / day after ovariectomy and induction of diabetes. Thereafter, sampling of spinal cord was carried out . The amount of BDNF was measured using RTPCR. The results showed that the injection of genistein significantly increased the amount of BDNF expression in the spinal cord when compared with the diabetic ovariectomy group (OVX.DI) and ovariecto my group (OVX) (p<0/05). Since BDNF is a member of the neurotrophic factor family, its expression is required for the regulation of growth, survival, and preservation of neurons and ovarian steroid . Therefore, genistein can be said to increase BDNF in the spinal cord.
Authors and Affiliations
Reihaneh Hoveida
Keywords
Related Articles
- EP ID EP247712
- DOI -
- Views 95
- Downloads 0
Coagulation profile in normal full-term neonate in the first week of life in Lagos-Nigeria
Normal reference values of haemostatic profile are frequently needed to assist in diagnosis and management of bleeding disorders. Because of the anatomical and physiological differences between neonates and adults, it is...
An anatomical study of variations in termination of brachial artery, with itsembryological basis and clinical significance
The brachial artery is the main artery of the arm. It begins as the continuation of 3rd part of axillary artery, at the level of inferior border of teres major muscle. It ends at the level of the neck of radius by dividi...
RPOB gene mutation in rifampicin resistant MRSA from foot infections of patients having diabetes mellitus
Staphylococcus aureus is an opportunistic bacterial pathogen associated with asymptomatic colonization of the skin and mucosal surfaces of normal humans. Staphylococcus aureus isolates are often multidrug resistant. Rifa...
Comparative efficacy of MIRU-VNTR and IS 6110-RFLP for differentiation of Mycobacterium tuberculosis isolates
The aim of this study was To characterize Mycobacterium tuberculosis isolates by MIRU-VNTR typing and IS 6110 – RFLP and to compare the efficiency of MIRU-VNTR typing and IS 6110 – RFLP in discriminating Mycobacterium tu...
Short Term Effects of Neurodynamic Stretching and Static Stretching Techniques on Hamstring Muscle Flexibility in Healthy Male Subjects
Flexibility is a key component of rehabilitation and inadequate muscle extensibility remains a commonly accepted factor for musculoskeletal disorders. Studies on the most optimal technique for improving muscle flexibilit...