Evaluating the effect of Myrtus communis on programmed cell death in hydatid cyst protoscolices
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2017, Vol 10, Issue 11
Abstract
Objective: To evaluate the possible involvement of programmed cell death strategy in hydatid cyst protoscolices following treatment with Myrtus communis (M. communis) as an herbal medicine. Methods: Protoscolices were aseptically collected from sheep liver hydatid cysts. Evaluating the effect of M. communis extract on programmed cell death and increased activity of caspases 3, 8, and 9 in hydatid cyst protoscolices was conducted by treating the protoscolices with different concentration (5, 50, and 100 mg/mL) of M. communis extract at 37 C and 5% CO2 for 4 h by using the Bradford test and ELISA commercial kits. Results: The extract of M. communis at all concentrations led to initiation of programmed cell death in protoscolices and this effect, was only significant at 50 and 100 mg/mL concentrations, compared to the negative control (P < 0.05). Also, the activity of caspases 3, 8, and 9 in hydatid cyst protoscolices, was shown that the extract at all 3 concentrations could only increase the activity of caspases 3 and 9. Moreover, a significant increase in the activity of caspase 3 was only observed at concentrations 50 and 100 mg/mL by 37.00% and 66.19% while a significant increase in the activity of caspase 9 at the same concentrations was observed by 20.89% and 63.67%, respectively (P < 0.05). Conclusions: The extract of M. communis at different concentrations could increase the activity of caspases 3 and 9 and caused programmed cell death in hydatid cyst protoscolices however, this effect was significant at high concentrations of the extract.
Authors and Affiliations
Mojtaba Shahnazi
Keywords
Related Articles
- EP ID EP246738
- DOI 10.1016/j.apjtm.2017.10.010
- Views 134
- Downloads 0
Clinical significance of dynamic detection for serum levels of MCP-1, TNF-a and IL-8 in patients with acute pancreatitis
Objective: To observe dynamic changes of levels of monocyte chemotactic protein-1 (MCP-1), tumor necrosis factor-a (TNF-a) and interleukin-8 (IL-8) in patients with acute pancreatitis and to investigate its evaluation va...
Latent tuberculosis infection among medical students in Malaysia
Objective: This study aimed to determine prevalence of latent tuberculosis infection among medical students and tuberculosis exposure at the health facilities. Methods: A cross-section of study year 1 (n=68) and year 5...
Lipase inhibitory activity of Lagenaria siceraria fruit as a strategy to treat obesity
Objective: To explore pancreatic lipase inhibitory activity under different extraction conditions in order to track the most potent extract. Methods: The methanolic extract and its fractions in solvents of increasing pol...
Control of animal brucellosis: The Malaysian experience
Brucellosis is a zoonotic disease characterized by reproductive failure in animals and undulent fever in humans. In cattle, it is caused by Brucella abortus while in goats by Brucella melitensis, the main cause of brucel...
In vitro and in vivo investigation of natural compounds from seed extract of Mucuna pruriens lacking L-DOPA for the treatment of erectile dysfunction
Objective: To investigate the biological effects of the Mucuna pruriens (M. pruriens) seed extracts that lacked L-DOPA, which was formerly reported as the active ingredient, on erectile dysfunction (ED) both in vitro and...