Evaluation and Comparision of Antimicrobial Activity of MTA to Antioxidant Mix Used As a Pulpotomy Agent- In Vitro Study.
Journal Title: International Journal of Dental Science and Innovative Research (IJDSIR) - Year 2019, Vol 2, Issue 1
Abstract
Introduction: Pulpotomy technique is most widely accepted clinical procedure for treating primary teeth with coronal pulp inflammation caused by caries with no involvement of the radicular pulp. Aim and Objective: Evaluation andComparision of antimicrobial activity of MTA to Antioxidant Mix used as a pulpotomy agent- In vitro study Materials and Methods: The test materials i.e MTA and antioxidant mix were manipulated strictly in accordance with the manufacturer's instructions. The antimicrobial activity of this material is evaluated by the agar diffusion method against two reference strains: E. faecalis (ATCC 29212), S. mutans (ATCC25175). Each material is evaluated at concentrations suggested by the manufacturer. Bacteria is diluted to obtain a suspension of approximately 5 × 108 colony forming units/ml, in sterile Trypticase Soy Broth (TSB). Microbial strains were confirmed by colony forming units and growth characteristics. E. faecalis is inoculated with sterile cotton swabs onto Meuller-Hinton agar plates and S. mutans is inoculated onto blood agar media. Wells 4 mm in diameter and 4 mm deep are prepared on plates with a copper puncher, and immediately filled with freshly manipulated test materials. After prediffusion of the test materials for 2 h at room temperature, all the plates are incubated at 37°C and evaluated at 24 h. Microbial inhibition zones are measured with a 0.5 mm precision ruler and the results are expressed as the mean and standard deviation. To compare the differences among MTA and antioxidant mix data are analyzed statistically by one-way analysis of variance (ANOVA) and Tukey's honest significant difference (HSD) post-hoc test, using Statistical Package for Social Sciences (SPSS) software version 21 (SPSS Inc, Chicago, IL, USA). Conclusion: MTA has better antimicrobial efficacy whereas Antioxidant mix as a new pulpotomy agent though biocompatible and cost effective than any other commercially available medicament does not show any antibacterical activity.
Authors and Affiliations
Dr. Heena Kagdi
Keywords
Related Articles
- EP ID EP580428
- DOI -
- Views 71
- Downloads 0
Properties of a Tricalcium Silicate Based Sealer.
Tricalcium silicate based materials are gaining popularity in various dental fields and the area of endodontics is no exception. Sealers based on tricalcium silicate aim at an interaction with the root canal wall, alkali...
Gender divergence in dimensions of tongue: A morphometric study.
The claimed testimony is a requisite part of forensic investigations. Anthropometry, the systematic collection and correlation of measurements of the human body is now one of the principal techniques of physical anthropo...
Radicular Cyst Followed By Pulpectomy in Deciduous Molar: A Case Report.
Radicular cysts are the most common odontogenic cystic lesion of inflammatory origin occurring in jaws. However, those arising from primary teeth are comparatively rare, comprising only 0.5 to 3.3...
Radiographic Profile of Symptomatic Impacted Mandibular Third Molars in the Arab Israeli Population.
Introduction: Impaction may be defined as the failure of complete eruption into a normal functional position of one tooth within normal time due to lack of space in the dental arch, caused by obstruction by another tooth...
Endodontic Disinfection Using Photodynamic Therapy with Indocyanine Green dye (ICG).
Aim: To analyze the antimicrobial effect of photodynamic therapy (PDT) in association with endodontic treatment. Methods: Thirty anterior teeth from patients were selected. Microbiological samples were taken 1) after ac...