Evaluation and Comparision of Antimicrobial Activity of MTA to Antioxidant Mix Used As a Pulpotomy Agent- In Vitro Study.
Journal Title: International Journal of Dental Science and Innovative Research (IJDSIR) - Year 2019, Vol 2, Issue 1
Abstract
Introduction: Pulpotomy technique is most widely accepted clinical procedure for treating primary teeth with coronal pulp inflammation caused by caries with no involvement of the radicular pulp. Aim and Objective: Evaluation andComparision of antimicrobial activity of MTA to Antioxidant Mix used as a pulpotomy agent- In vitro study Materials and Methods: The test materials i.e MTA and antioxidant mix were manipulated strictly in accordance with the manufacturer's instructions. The antimicrobial activity of this material is evaluated by the agar diffusion method against two reference strains: E. faecalis (ATCC 29212), S. mutans (ATCC25175). Each material is evaluated at concentrations suggested by the manufacturer. Bacteria is diluted to obtain a suspension of approximately 5 × 108 colony forming units/ml, in sterile Trypticase Soy Broth (TSB). Microbial strains were confirmed by colony forming units and growth characteristics. E. faecalis is inoculated with sterile cotton swabs onto Meuller-Hinton agar plates and S. mutans is inoculated onto blood agar media. Wells 4 mm in diameter and 4 mm deep are prepared on plates with a copper puncher, and immediately filled with freshly manipulated test materials. After prediffusion of the test materials for 2 h at room temperature, all the plates are incubated at 37°C and evaluated at 24 h. Microbial inhibition zones are measured with a 0.5 mm precision ruler and the results are expressed as the mean and standard deviation. To compare the differences among MTA and antioxidant mix data are analyzed statistically by one-way analysis of variance (ANOVA) and Tukey's honest significant difference (HSD) post-hoc test, using Statistical Package for Social Sciences (SPSS) software version 21 (SPSS Inc, Chicago, IL, USA). Conclusion: MTA has better antimicrobial efficacy whereas Antioxidant mix as a new pulpotomy agent though biocompatible and cost effective than any other commercially available medicament does not show any antibacterical activity.
Authors and Affiliations
Dr. Heena Kagdi
Keywords
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