EVALUATION AND COMPARISON OF CYTOTOXIC EFFECT OF VILAZODONE HYDROCHLORIDE WITH 5-FLUOROURACIL IN HT-29 BOWEL CANCER CELL LINE
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 2
Abstract
Objectives: Vilazodone hydrochloride is a novel selective serotonin reuptake inhibitor (SSRI) used to treat major depressive disorders. There are only sparse data available to know about the SSRI’s and its association with colon cancer. This study aims to evaluate and compare the in vitro cytotoxic effect of vilazodone with 5-fluorouracil (5-FU) in HT-29 cell line. Methods: Cell viability was tested by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay (Mosmann, 1983). Test sample and standard drug in variable concentrations were added to the HT-29 cell lines for incubation over 24 h under ideal conditions. After washing the test and standard drug sample from the well with saline, MTT was added and incubated for 4 h. Dimethyl sulfoxide of 1 ml was added in all wells after incubation with MTT. The absorbance at 570 nm was measured with an ultraviolet - spectrophotometer. Results: The values were tabulated, and the graph was plotted to find the IC-50 value (inhibitory concentration at 50%) which was struck at 28.5 μg/ml and12. 8 μg/ml for vilazodone hydrochloride and 5-FU, respectively. Conclusion: The results show that vilazodone hydrochloride has good anticancer property comparable with 5-FU, which would probably play a role as a cytotoxic agent in tumor cells. The proposed mechanism of action could be by activation of caspase-3 enzyme, thereby increasing apoptosis and indicates its use in coexisting depression and colon carcinoma. Other mechanism includes suppression of oncogene p53, which can be confirmed by future studies.
Authors and Affiliations
LATHA PRIYA A, ANUSHA D, DARLING CHELLATHAI K, HEMALATHA A, JEGAN MOHAN Y
Keywords
Related Articles
- EP ID EP608747
- DOI -
- Views 115
- Downloads 0
THE EFFECT OF EXTRACT OF DATE PALM FRUIT (PHOENIX DACTYLIFERA L.) ON FERTILITY IN MALE MICE (MUS MUSCULUS L.)
Objective: Date palm is one of the plants that have used empirically to increase fertility. Ethanol induction can cause sperm damage in male mice. Sperm damage is one of the infertility agents. The aim of this study was...
PATIENTS’ KNOWLEDGE, ATTITUDES, AND PRACTICES ON UNCOMPLICATED MALARIA MANAGEMENT IN PLATEAU STATE, NORTH-CENTRAL NIGERIA
Objectives: This study aimed to assess patients’ knowledge, attitudes, and practices (KAP) on uncomplicated malaria management in primary health-care (PHC) facilities of Plateau state, Nigeria. Methods: A validated self...
A SYNTHESIS AND REVIEW OF MEDICINAL USES, PHYTOCHEMISTRY AND BIOLOGICAL ACTIVITIES OF HELICHRYSUM ODORATISSIMUM (L.) SWEET
Helichrysum odoratissimum is an important medicinal plant species in tropical Africa. The current study critically reviewed the medicinal uses, phytochemistry and biological activities of H. odoratissimum. Information on...
IN VITRO ANALYSIS: THE ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ZINC OXIDE NANOPARTICLES FROM CURCUMA LONGA
Objective: Curcuma longa is a known natural medicine for inflammation from ancient times. It has a low absorption rate and poor solubility. Hence, it is used for the green synthesis of nanoparticles. Zinc oxide nanoparti...
THE STUDY OF POTENTIALITY OF PICRORHIZA KURROA ROOT PROTEINS TO INHIBIT FREE RADICALS AND Α-AMYLASE ENZYME
 Objective: To prevent oxidative damage to cells caused by free radicals that are responsible for many diseases, such as atherosclerosis, diabetesand cancer. Picrorhiza kurroa (Katuki) is an ayurvedic medicinal plant us...