Evaluation and optimization of chitosan derivatives-based gene delivery system via kidney epithelial cells
Journal Title: Advanced Pharmaceutical Bulletin - Year 2012, Vol 2, Issue 1
Abstract
[sub][/sub]Purpose: Non-viral vectors have been widely proposed as safer alternatives to viral vectors, and cationic polymers have gained increasing attention because they can form self-assembly with DNA. Chitosan is also considered to be a good candidate for gene delivery systems, since it is already known as a biocompatible, biodegradable, and low toxic material with high cationic potential. However, low solubility and transfection efficiency need to be overcome prior to clinical trial. In this work, we focus on alkyl modified chitosan which might be useful in DNA condensing and efficient gene delivery. Methods: N, N- Diethyl N- Methyl (DEMC) and N- Triethyl Chitosan (TEC) were synthesized from chitosan polymer. In order to optimize the polymers for gene delivery, we used FITC-dextran (FD). Then the optimized polymer concentrations were used for gene delivery. Fluorescent microscope was used, in order to evaluate the polymers’ efficiency for gene delivery to human embryonic kidney epithelial cells (HEK 293T). Results: This modification increased chitosan’s positive charge, thus these chitosan derivatives spontaneously formed complexes with FD, green fluorescence protein plasmid DNA (pEGFP), red fluorescence protein plasmid DNA (pJred) and fluorescent labeled miRNA .Results gained from fluorescent microscope showed that TEC and DEMC were able to transfer FD, DNA and miRNA (micro RNA) to HEK cell line. Conclusion: We conclude that these chitosan derivatives present suitable characteristics to be used as non-viral gene delivery vectors to epithelial cells.
Authors and Affiliations
S. Safari, M. H. Zarrintan, M. Soleimani, F. A. Dorkoosh, H. Akbari, B. Larijani
Keywords
Related Articles
- EP ID EP119942
- DOI 10.5681/apb.2012.002
- Views 114
- Downloads 0
Determination of comparative minimum inhibitory concentration (MIC) of bacteriocins produced by enterococci for selected isolates of multi-antibiotic resistant Enterococcus spp.
Introduction: The occurrence of multi-antibiotic resistance among enterococci is a prevalent clinical problem worldwide and continues to get serious due to the lack of efficient therapeutic options by the time. In this r...
Novel PH Sensitive Nanocarrier Agents Based on Citric Acid Dendrimers Containing Conjugated β-Cyclodextrins
Introduction: In this work, the use of β-cyclodextrine (β-CD)-modified dendrimers as a nanocapsule with a biocompatible shell have studied. β-CD-modifieddendrimers have designed and synthesized to enhance the loading cap...
Skin Infection Management Using Novel Antibacterial Agents
Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA) cause difficulties in the management of skin and soft tissue infections and have led to morbidity and mortality in hospital-acquired infection...
Phytochemical Screening and Anti-nociceptive Properties of the Ethanolic Leaf Extract of Trema Cannabina Lour
Purpose: The present study was designed to investigate the anti-nociceptive activity of ethanolic leaf extract of Trema cannabina Lour (family: Cannabaceae) in experimental animal models. Methods: The anti-nociceptive ac...
Simultaneous Determination of Tetracyclines Residues in Bovine Milk Samples by Solid Phase Extraction and HPLC-FL Method
Introduction: Tetracyclines (TCs) are widely used in animal husbandry and their residues in milk may resultin harmful effects on human. The aim of this study was to investigate the presence of TCs residues in various bov...