Evaluation In-Vitro Antioxidant, Antimicrobial and Laxative Potential of Ethanol Leaves Extract of Synedrellanodiflora (L) Gaertn on Wistar Albino Rats
Journal Title: Scholars Academic Journal of Pharmacy - Year 2017, Vol 6, Issue 9
Abstract
Synedrellanodiflora (L) Gaertn, commonly known as Pig grass, Cinderella weed, node weed is a species of flowering plant in the family of Asteraceae. It is a medicinal plant mostly used in the herbal medicine to cure leprosy, swelling, constipation, dysentery and diarrhea. Ethanol leaves extract of Synedrellanodiflorawere investigated on in-vitro antioxidant, antimicrobial and laxatives activities in albino rats and were compared with the standard drugs Ascorbic acid(100mg/kg), Ciprofloxacin(40mg/kg) and Ducox(300mg/kg) respectively. The extract were administered based on high and low doses, 2000,1000,500,250,125 and 62.5mg/ml. Different models such as DPPH radical scavenging activity, Nitric oxide scavenging activity and Anti-lipid peroxidation activity were used to determine antioxidant activity. Healthy adult wistar albino rats of both sex weighing 150-200g was used to determine laxative effect of the ethanol leaves extract, for the antimicrobial activity, clinical strains of microorganism used for the study includes: E. coli, Proteus mirabilis, Pseudomonaaeruginose, Staphylococcus aureus, Salmonella typi and Klebsiella pneumonia. The agar disc diffusion method was used and zone of inhibition recorded also minimum inhibitory concentration was determined. Result, the IC50 for nitric oxide yielded 70.70+- 3.52 at 200ug/ml, for anti-lipid peroxidation it yielded 53.97+-5.08 at 100ug/ml, but for DPPH, the results were less when compared with the standard drug(Ascorbic acid). The antimicrobial activity results at different graded concentrations of 2000, 1000, 500, 250, 125 and 62.5mg/ml, showed that the ethanol extract containing 2000mg/ml has the highest mean+-SD zone of inhibition against the clinical pathogens while the disc containing 62.5mg/ml revealed least inhibitory zones. The laxatives activity, there was a dose dependent increase in the faecal output of the rats when compared to the control group. The effect of the extract shows a similar trend to that of the standard drug which justified its medicinal potency. Keywords:Syndrellanodiflora, Antimicrobial, Nitric oxide, DPPH, Lipid peroxidation, Laxative.
Authors and Affiliations
M. A Jimoh, O. D Omodamiro
Keywords
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