Evaluation of 5-year results of the inversion of inv(16) CBFBETA-MYH11 with real time RT-PCR in patients prediagnosed as AML
Journal Title: Ege Tıp Dergisi - Year 2015, Vol 54, Issue 1
Abstract
Aim: In this study, it was aimed to evaluate the analysis of inv16 blood and bone marrow samples quantitively by reverse transcriptase polymerase chain reaction (RT-PCR) method of 402 patients (322 adult, 80 children) prediagnosed as acute myeloid leukemia (AML), who were admitted to Ege University Faculty of Medicine, Department of Medical Biology between years 2009-2013. Materials and Methods: cDNA’s were obtained following the total RNA/mRNA isolation which were taken from patients’ blood and bone marrow samples and quantitaion of inv16 were performed by reverse transcriptase RT-PCR method at LightCycler2. Results: Four hundred and two cases were evaluated in terms of inv(16) CBFBETA-MYH11 inversion. As a result of the analysis, a total of 19 patient were determined inv16 positivity includes 12 adult (% 4) and 7 children (9%). Conclusion: Inv16 of quantitative analysis is supported to be an effective method for clinical evaluation of patients with AML, regarding remission of disease and implimentation of the treatment protocols toward achieving remission. More studies with AML patients are required for confirmation of the results.
Authors and Affiliations
Aslı TETİK VARDARLI, Burçin TEZCANLI KAYMAZ, Vildan BOZOK ÇETİNTAŞ, Sunde YILMAZ SÜSLÜER, Duygu AYGÜNEŞ, Ayşegül DALMIZRAK, Çağdaş AKTAN, Ali Şahin KÜÇÜKASLAN, Tuğçe BALCI, Çağla KAYABAŞ, Besra ÖZMEN YELKEN, Nur SELVİ GÜNEL, Çığır BİRAY AVCI, Buket KOSOVA, Zuhal EROĞLU, Serap AKSOYLAR, Nazan ÇETİNGÜL, Can BALKAN, Deniz YILMAZ, Yeşim AYDINOK, Kaan KAVAKLI, Mahmut TÖBÜ, Murat TOMBULOĞLU, Filiz BÜYÜKKEÇECİ, Fahri ŞAHİN, Güray SAYDAM, Cumhur Gündüz
Keywords
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