Evaluation of α-Amylase Inhibitory Potentials of Sida acuta, Tithonia diversifolia and Chromolaena odorata Leaf Extracts
Journal Title: Journal of Advances in Biology & Biotechnology - Year 2017, Vol 14, Issue 4
Abstract
Aim: To investigate the inhibitory effect of Sida acuta, Tithonia diversifolia and Chromolaena odorata leaf extracts on the activity of carbohydrate hydrolyzing enzyme (α-amylase) and to provide scientific validation of their folk use in diabetes treatment. Study Design: The α-amylase inhibition assay was carried out as a measure of the anti-diabetic potentials of Sida acuta, Tithonia diversifolia and Chromolaena odorata. Place and Duration: Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria (January – November, 2016). Methodology: Cold water, hot water and ethanolic extracts of Sida acuta, Tithonia diversifolia and Chromolaena odorata were obtained. Standard method was employed in the α-amylase inhibitory assays (3, 5-dinitrosalicylic acid (DNSA) method). Results: The results revealed that the extracts had a dose dependent prevention of digestion of carbohydrates by inhibiting α-amylase. Ethanolic extracts of T. diversifolia, C. odorata and S. acuta gave the highest inhibitory activities against α-amylase with 41.02% (IC50 0.754 mg/ml), 43.67% (IC50 0.619 mg/ml) and 45.72% (IC50 0.604 mg/ml) maximum inhibition at a concentration of 500 µg/ml respectively. Conclusion: It can be concluded from this study that extracts of these plants possessed great potential as anti-diabetic agents by inhibiting α-amylase. The presence of phytochemicals like flavonoids, saponins, and tannins might have contributed greatly to the inhibitory activities exhibited by these plant extracts.
Authors and Affiliations
Kemi Feyisayo Akinwunmi, Abiodun Akeem Ajiboye, Omolara Olajumoke Ojo
Keywords
Related Articles
- EP ID EP318126
- DOI 10.9734/JABB/2017/35148
- Views 74
- Downloads 0
Performance, Apparent Nutrient Digestibility and Cost Benefit of West African Dwarf Goats Fed Dietary Levels of Moringa oleifera Leaf Meal
Aims: To determine the performance, apparent nutrient digestibility and cost benefit of West African Dwarf goats fed dietary levels of Moringa oleifera leaf meal diets. Study Design: Completely randomized design and Lati...
Optimization of Conditions for Biosorption of Chromium and Cadmium by Shell of Anodontoides ferussacianus
The biosorption of chromium [Cr (VI)] and cadmium [Cd (II)] ions using dead biomass of bivalve mollusk shell, Anodontoides ferussacianus was studied. Effect of various physico-chemical parameters was determined. Optimum...
Computational Analysis of Evolutionary Relationship of a Family of Cold Shock Proteins in Ten Mammalian Species
Aims: This study was carried out to evaluate the evolutionary relationship of a family of cold shock proteins (CSP) in ten mammalian species using bioinformatics tools and soft wares such as Genbank, FASTA, BLAST and MEG...
Partial Purification and Characterization of Angiotensin Converting Enzyme Inhibitory Alkaloids and Flavonoids from the Leaves and Seeds of Moringa oleifera
Aim: In the present study, the alkaloid, flavonoid and saponin-rich extracts of the leaves and seeds of Moringa oleifera were partially-purified and the ACE inhibitory activities and patterns of inhibition determined. Me...
Evaluation of Bacillus thuringiensis Snkr10 as a Potent Plant Growth Promoting Phyllospheric Bacterium Isolated from Spinach (Spinacia oleracea) Plants
The phyllospheric microorganism used leaf surface of plants as habitat and help to maintain the plants growth by behaving as plant growth promoting bacteria (PGPB). SNKr10 is a gram positive and rod shaped spinach phyllo...