Evaluation of α-Amylase Inhibitory Potentials of Sida acuta, Tithonia diversifolia and Chromolaena odorata Leaf Extracts
Journal Title: Journal of Advances in Biology & Biotechnology - Year 2017, Vol 14, Issue 4
Abstract
Aim: To investigate the inhibitory effect of Sida acuta, Tithonia diversifolia and Chromolaena odorata leaf extracts on the activity of carbohydrate hydrolyzing enzyme (α-amylase) and to provide scientific validation of their folk use in diabetes treatment. Study Design: The α-amylase inhibition assay was carried out as a measure of the anti-diabetic potentials of Sida acuta, Tithonia diversifolia and Chromolaena odorata. Place and Duration: Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria (January – November, 2016). Methodology: Cold water, hot water and ethanolic extracts of Sida acuta, Tithonia diversifolia and Chromolaena odorata were obtained. Standard method was employed in the α-amylase inhibitory assays (3, 5-dinitrosalicylic acid (DNSA) method). Results: The results revealed that the extracts had a dose dependent prevention of digestion of carbohydrates by inhibiting α-amylase. Ethanolic extracts of T. diversifolia, C. odorata and S. acuta gave the highest inhibitory activities against α-amylase with 41.02% (IC50 0.754 mg/ml), 43.67% (IC50 0.619 mg/ml) and 45.72% (IC50 0.604 mg/ml) maximum inhibition at a concentration of 500 µg/ml respectively. Conclusion: It can be concluded from this study that extracts of these plants possessed great potential as anti-diabetic agents by inhibiting α-amylase. The presence of phytochemicals like flavonoids, saponins, and tannins might have contributed greatly to the inhibitory activities exhibited by these plant extracts.
Authors and Affiliations
Kemi Feyisayo Akinwunmi, Abiodun Akeem Ajiboye, Omolara Olajumoke Ojo
Keywords
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- EP ID EP318126
- DOI 10.9734/JABB/2017/35148
- Views 92
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