Evaluation of Acute Toxicity and Hypoglycaemic and Hypolipidaemic Effects of Cyathula prostata (Linn.) Blume Weeds on Adult Rats
Journal Title: Journal of Pharmaceutical Research International - Year 2016, Vol 9, Issue 5
Abstract
Objectives: The study aimed at evaluating the acute toxicity and hypoglycaemic and hypolipidaemic effects of Cyathula prostata plant used locally in the treatment of various diseases including diabetes. Materials and Methods: The toxicity of the extract was evaluated in Swiss albino mice by feeding the animals with the graded doses of the extract between 1.0 to 20.0 g/kg body weight (bwt) and continuously observed for the first 4 h, then hourly for the next 12 hand 6 hourly for the next 56 h (72 h, acute toxicity). Diabetes was induced in the male and female Wistar rats with alloxan monohydrate, at the dose of 150 mg/kg, dissolved in normal saline and administered intraperitoneally (i.p). The plasma glucose levels of the induced animals were monitored with a Glucometer (MERCK) after 72 h. The animals with plasma glucose level ≥200 mg/dl were classified as diabetic and were included in the study. The diabetic animals were randomly distributed into five groups of 5 animals each. The first group was normal not treated but received acacia 2% w/v solution. The second group was induced but not treated while the remaining four groups were treated with the reference drug, glibenclamide, (600 µg-1 kg bwt)/ the extract in graded doses of 75, 150 and 250 mg/kg bwt respectively for 30 days. Results: The animals that received 20 g/kg bwt of the extract survived beyond 24 hrs, therefore the LD50 could be assumed to be above 20 g -1 kg bwt. The plasma level of alanine aminotransferase (ALT) was found to have significantly (p≤0.05) increased in the extract treated compared to the negative control whereas aspartate aminotransferase (AST) level showed marked decrease with dose. However, hepatic tissue histology at the highest extract dose treatment showed mild portal hepatitis with hepatocytes vacuolization and sinusoidal congestion. There were no significant changes (p≥0.05) in protein and creatinine levels. There were significant (p≤0.05) reduction in the plasma glucose, cholesterol, triglycerides and LDL levels while a significant increase in HDL levels was observed. The tissue histology of pancreas showed significant survivor of beta cells in the extract treated groups. Conclusion: The extract was observed to have beneficial effect on alloxan-damaged pancreas as beta cells showed signs of recovery.
Authors and Affiliations
S. O. Ogbonnia, G. O. Mbaka, O. E. Akinsande, D. A. Otah, T. A. Ayeni
Keywords
Related Articles
- EP ID EP342308
- DOI 10.9734/BJPR/2016/20433
- Views 84
- Downloads 0
A Comparative Study of Analgesic, Antipyretic and Anti-inflammatory Effect of Ethanolic Extract of Trigonella foenum-graecum with Indomethacin and Diclofenac Sodium
Many drugs are available for the treatment of pain and inflammation but their utility is associated with various adverse effects. To eliminate such adverse effects natural products having medicinal effects are been given...
Nephroprotective Effect of Phoenix reclinata Total Crude Root Extract on Tenofovir Induced Kidney Damage in Wistar Albino Rats
Tenofovir (TDF), used in combination with other antiretroviral agents, is an effective therapy for HIV infection. However, prolonged use of this drug is limited by a life threatening nephrotoxicity. Phoenix reclinata is...
In-vitro Antioxidant and Cytotoxic Activity of Crude Extracts of Pleurotus highking, a Potential Oyster Mushroom
Contemporary civilization is facing more than thousands of disorders accompanied with free radicals. Antioxidants from edible and non-edible mushrooms are gaining importance to fight these disorders. The current investig...
Analgesic, Anti-inflammatory and CNS Depressant Activities of the Methanolic Extract of Abelmoschus esculentus Linn. Seed in Mice
Aims: The study was carried out to assess the analgesic, anti-inflammatory and CNS depressant activity of the methanolic extract of Abelmoschus esculentus Linn. Seeds. Study Design: The Present study was designed to obse...
In vivo Antioxidant Activity and Anti Hyperglycemic Relevant Enzyme Inhibition Properties of Petroleum Ether Extract of Traditionaly Processed Ocimum canum Leaves
Objective: To investigate the antihyperglycemic and antioxidant properties of the petroleum ether extract of Ocimum canum leaves in streptozotocin-induced diabetic rats. Place and Duration of Study: Pinacle Biomedical Re...